J. Mason Earles

Devin A. Rippner, Pranav Raja, J. Mason Earles et al.

X-ray micro-computed tomography (X-ray microCT) has enabled the characterization of the properties and processes that take place in plants and soils at the micron scale. Despite the widespread use of this advanced technique, major limitations in both hardware and software limit the speed and accuracy of image processing and data analysis. Recent advances in machine learning, specifically the application of convolutional neural networks to image analysis, have enabled rapid and accurate segmentation of image data. Yet, challenges remain in applying convolutional neural networks to the analysis of environmentally and agriculturally relevant images. Specifically, there is a disconnect between the computer scientists and engineers, who build these AI/ML tools, and the potential end users in agricultural research, who may be unsure of how to apply these tools in their work. Additionally, the computing resources required for training and applying deep learning models are unique, more common to computer gaming systems or graphics design work, than to traditional computational systems. To navigate these challenges, we developed a modular workflow for applying convolutional neural networks to X-ray microCT images, using low-cost resources in Googles Colaboratory web application. Here we present the results of the workflow, illustrating how parameters can be optimized to achieve best results using example scans from walnut leaves, almond flower buds, and a soil aggregate. We expect that this framework will accelerate the adoption and use of emerging deep learning techniques within the plant and soil sciences.

Alexander G. Olenskyj, Brent S. Sams, Zhenghao Fei et al.

Yield estimation is a powerful tool in vineyard management, as it allows growers to fine-tune practices to optimize yield and quality. However, yield estimation is currently performed using manual sampling, which is time-consuming and imprecise. This study demonstrates the application of proximal imaging combined with deep learning for yield estimation in vineyards. Continuous data collection using a vehicle-mounted sensing kit combined with collection of ground truth yield data at harvest using a commercial yield monitor allowed for the generation of a large dataset of 23,581 yield points and 107,933 images. Moreover, this study was conducted in a mechanically managed commercial vineyard, representing a challenging environment for image analysis but a common set of conditions in the California Central Valley. Three model architectures were tested: object detection, CNN regression, and transformer models. The object detection model was trained on hand-labeled images to localize grape bunches, and either bunch count or pixel area was summed to correlate with grape yield. Conversely, regression models were trained end-to-end to predict grape yield from image data without the need for hand labeling. Results demonstrated that both a transformer as well as the object detection model with pixel area processing performed comparably, with a mean absolute percent error of 18% and 18.5%, respectively on a representative holdout dataset. Saliency mapping was used to demonstrate the attention of the CNN model was localized near the predicted location of grape bunches, as well as on the top of the grapevine canopy. Overall, the study showed the applicability of proximal imaging and deep learning for prediction of grapevine yield on a large scale. Additionally, the end-to-end modeling approach was able to perform comparably to the object detection approach while eliminating the need for hand-labeling.

Lars Lundqvist, Earl Ranario, Hamid Kamangir et al.

Vision foundation models (VFMs) offer the promise of zero-shot object detection without task-specific training data, yet their performance in complex agricultural scenes remains highly sensitive to text prompt construction. We present a systematic prompt optimization framework evaluating four open-vocabulary detectors -- YOLO World, SAM3, Grounding DINO, and OWLv2 -- for cowpea flower and pod detection across synthetic and real field imagery. We decompose prompts into eight axes and conduct one-factor-at-a-time analysis followed by combinatorial optimization, revealing that models respond divergently to prompt structure: conditions that optimize one architecture can collapse another. Applying model-specific combinatorial prompts yields substantial gains over a naive species-name baseline, including +0.357 mAP@0.5 for YOLO World and +0.362 mAP@0.5 for OWLv2 on synthetic cowpea flower data. To evaluate cross-task generalization, we use an LLM to translate the discovered axis structure to a morphologically distinct target -- cowpea pods -- and compare against prompting using the discovered optimal structures from synthetic flower data. Crucially, prompt structures optimized exclusively on synthetic data transfer effectively to real-world fields: synthetic-pipeline prompts match or exceed those discovered on labeled real data for the majority of model-object combinations (flower: 0.374 vs. 0.353 for YOLO World; pod: 0.429 vs. 0.371 for SAM3). Our findings demonstrate that prompt engineering can substantially close the gap between zero-shot VFMs and supervised detectors without requiring manual annotation, and that optimal prompts are model-specific, non-obvious, and transferable across domains.

Heesup Yun, Isaac Kazuo Uyehara, Ioannis Droutsas et al.

Three-dimensional (3D) procedural plant architecture models have emerged as an important tool for simulation-based studies of plant structure and function, extracting plant architectural parameters from field measurements, and for generating realistic plants in computer graphics. However, measuring the architectural parameters and nested structures for these models at the field scales remains prohibitively labor-intensive. We present a novel algorithm that generates a 3D plant architecture from an image, creating a functional structural plant model that reflects organ-level geometric and topological parameters and provides a more comprehensive representation of the plant's architecture. Instead of using 3D sensors or processing multi-view images with computer vision to obtain the 3D structure of plants, we proposed a method that generates token sequences that encode a procedural definition of plant architecture. This work used only synthetic images for training and testing, with exact architectural parameters known, allowing testing of the hypothesis that organ-level architectural parameters could be extracted from image data using a vision-language model (VLM). A synthetic dataset of cowpea plant images was generated using the Helios 3D plant simulator, with the detailed plant architecture encoded in XML files. We developed a plant architecture tokenizer for the XML file defining plant architecture, converting it into a token sequence that a language model can predict. The model achieved a token F1 score of 0.73 during teacher-forced training. Evaluation of the model was performed through autoregressive generation, achieving a BLEU-4 score of 94.00% and a ROUGE-L score of 0.5182. This led to the conclusion that such plant architecture model generation and parameter extraction were possible from synthetic images; thus, future work will extend the approach to real imagery data.

Earl Ranario, Lars Lundqvist, Heesup Yun et al.

Semantically consistent cross-domain image translation facilitates the generation of training data by transferring labels across different domains, making it particularly useful for plant trait identification in agriculture. However, existing generative models struggle to maintain object-level accuracy when translating images between domains, especially when domain gaps are significant. In this work, we introduce AGILE (Attention-Guided Image and Label Translation for Efficient Cross-Domain Plant Trait Identification), a diffusion-based framework that leverages optimized text embeddings and attention guidance to semantically constrain image translation. AGILE utilizes pretrained diffusion models and publicly available agricultural datasets to improve the fidelity of translated images while preserving critical object semantics. Our approach optimizes text embeddings to strengthen the correspondence between source and target images and guides attention maps during the denoising process to control object placement. We evaluate AGILE on cross-domain plant datasets and demonstrate its effectiveness in generating semantically accurate translated images. Quantitative experiments show that AGILE enhances object detection performance in the target domain while maintaining realism and consistency. Compared to prior image translation methods, AGILE achieves superior semantic alignment, particularly in challenging cases where objects vary significantly or domain gaps are substantial.

Earl Ranario, Ismael Mayanja, Heesup Yun et al.

Accurate plant segmentation in thermal imagery remains a significant challenge for high throughput field phenotyping, particularly in outdoor environments where low contrast between plants and weeds and frequent occlusions hinder performance. To address this, we present a framework that leverages synthetic RGB imagery, a limited set of real annotations, and GAN-based cross-modality alignment to enhance semantic segmentation in thermal images. We trained models on 1,128 synthetic images containing complex mixtures of crop and weed plants in order to generate image segmentation masks for crop and weed plants. We additionally evaluated the benefit of integrating as few as five real, manually segmented field images within the training process using various sampling strategies. When combining all the synthetic images with a few labeled real images, we observed a maximum relative improvement of 22% for the weed class and 17% for the plant class compared to the full real-data baseline. Cross-modal alignment was enabled by translating RGB to thermal using CycleGAN-turbo, allowing robust template matching without calibration. Results demonstrated that combining synthetic data with limited manual annotations and cross-domain translation via generative models can significantly boost segmentation performance in complex field environments for multi-model imagery.