Lydia A. Schoenpflug

Lydia A. Schoenpflug, Maxime W. Lafarge, Anja L. Frei et al.

Automating tissue segmentation and tumor detection in histopathology images of colorectal cancer (CRC) is an enabler for faster diagnostic pathology workflows. At the same time it is a challenging task due to low availability of public annotated datasets and high variability of image appearance. The semi-supervised learning for CRC detection (SemiCOL) challenge 2023 provides partially annotated data to encourage the development of automated solutions for tissue segmentation and tumor detection. We propose a U-Net based multi-task model combined with channel-wise and image-statistics-based color augmentations, as well as test-time augmentation, as a candidate solution to the SemiCOL challenge. Our approach achieved a multi-task Dice score of .8655 (Arm 1) and .8515 (Arm 2) for tissue segmentation and AUROC of .9725 (Arm 1) and 0.9750 (Arm 2) for tumor detection on the challenge validation set. The source code for our approach is made publicly available at https://github.com/lely475/CTPLab_SemiCOL2023.

Lydia A. Schoenpflug, Viktor H. Koelzer

Detecting and classifying cells in histopathology H\&E stained whole-slide images is a core task in computational pathology, as it provides valuable insight into the tumor microenvironment. In this work we investigate the impact of ground truth formats on the models performance. Additionally, cell-tissue interactions are considered by providing tissue segmentation predictions as input to the cell detection model. We find that a "soft", probability-map instance segmentation ground truth leads to best model performance. Combined with cell-tissue interaction and test-time augmentation our Soft Cell-Tissue-Model (SoftCTM) achieves 0.7172 mean F1-Score on the Overlapped Cell On Tissue (OCELOT) test set, achieving the third best overall score in the OCELOT 2023 Challenge. The source code for our approach is made publicly available at https://github.com/lely475/ocelot23algo.

JaeWoong Shin, Jeongun Ryu, Aaron Valero Puche et al.

Pathologists routinely alternate between different magnifications when examining Whole-Slide Images, allowing them to evaluate both broad tissue morphology and intricate cellular details to form comprehensive diagnoses. However, existing deep learning-based cell detection models struggle to replicate these behaviors and learn the interdependent semantics between structures at different magnifications. A key barrier in the field is the lack of datasets with multi-scale overlapping cell and tissue annotations. The OCELOT 2023 challenge was initiated to gather insights from the community to validate the hypothesis that understanding cell and tissue (cell-tissue) interactions is crucial for achieving human-level performance, and to accelerate the research in this field. The challenge dataset includes overlapping cell detection and tissue segmentation annotations from six organs, comprising 673 pairs sourced from 306 The Cancer Genome Atlas (TCGA) Whole-Slide Images with hematoxylin and eosin staining, divided into training, validation, and test subsets. Participants presented models that significantly enhanced the understanding of cell-tissue relationships. Top entries achieved up to a 7.99 increase in F1-score on the test set compared to the baseline cell-only model that did not incorporate cell-tissue relationships. This is a substantial improvement in performance over traditional cell-only detection methods, demonstrating the need for incorporating multi-scale semantics into the models. This paper provides a comparative analysis of the methods used by participants, highlighting innovative strategies implemented in the OCELOT 2023 challenge.