Vinil Polepalli

Vinil Polepalli

Pan-cancer classification using transcriptomic (RNA-Seq) data can inform tumor subtyping and therapy selection, but is challenging due to extremely high dimensionality and limited sample sizes. In this study, we propose a novel deep learning framework that uses a class-conditional variational autoencoder (cVAE) to augment training data for pan-cancer gene expression classification. Using 801 tumor RNA-Seq samples spanning 5 cancer types from The Cancer Genome Atlas (TCGA), we first perform feature selection to reduce 20,531 gene expression features to the 500 most variably expressed genes. A cVAE is then trained on this data to learn a latent representation of gene expression conditioned on cancer type, enabling the generation of synthetic gene expression samples for each tumor class. We augment the training set with these cVAE-generated samples (doubling the dataset size) to mitigate overfitting and class imbalance. A two-layer multilayer perceptron (MLP) classifier is subsequently trained on the augmented dataset to predict tumor type. The augmented framework achieves high classification accuracy (~98%) on a held-out test set, substantially outperforming a classifier trained on the original data alone. We present detailed experimental results, including VAE training curves, classifier performance metrics (ROC curves and confusion matrix), and architecture diagrams to illustrate the approach. The results demonstrate that cVAE-based synthetic augmentation can significantly improve pan-cancer prediction performance, especially for underrepresented cancer classes.

Vinil Polepalli

The invasive spotted lanternfly (SLF) poses a significant threat to agriculture and ecosystems, causing widespread damage. Current control methods, such as egg scraping, pesticides, and quarantines, prove labor-intensive, environmentally hazardous, and inadequate for long-term SLF suppression. This research introduces LanternNet, a novel autonomous robotic Hub-and-Spoke system designed for scalable detection and suppression of SLF populations. A central, tree-mimicking hub utilizes a YOLOv8 computer vision model for precise SLF identification. Three specialized robotic spokes perform targeted tasks: pest neutralization, environmental monitoring, and navigation/mapping. Field deployment across multiple infested sites over 5 weeks demonstrated LanternNet's efficacy. Quantitative analysis revealed significant reductions (p < 0.01, paired t-tests) in SLF populations and corresponding improvements in tree health indicators across the majority of test sites. Compared to conventional methods, LanternNet offers substantial cost advantages and improved scalability. Furthermore, the system's adaptability for enhanced autonomy and targeting of other invasive species presents significant potential for broader ecological impact. LanternNet demonstrates the transformative potential of integrating robotics and AI for advanced invasive species management and improved environmental outcomes.