Ron Appel

Kevin C. Zhou, Mark Harfouche, Maxwell Zheng et al.

We present a large-scale computational 3D topographic microscope that enables 6-gigapixel profilometric 3D imaging at micron-scale resolution across $>$110 cm$^2$ areas over multi-millimeter axial ranges. Our computational microscope, termed STARCAM (Scanning Topographic All-in-focus Reconstruction with a Computational Array Microscope), features a parallelized, 54-camera architecture with 3-axis translation to capture, for each sample of interest, a multi-dimensional, 2.1-terabyte (TB) dataset, consisting of a total of 224,640 9.4-megapixel images. We developed a self-supervised neural network-based algorithm for 3D reconstruction and stitching that jointly estimates an all-in-focus photometric composite and 3D height map across the entire field of view, using multi-view stereo information and image sharpness as a focal metric. The memory-efficient, compressed differentiable representation offered by the neural network effectively enables joint participation of the entire multi-TB dataset during the reconstruction process. To demonstrate the broad utility of our new computational microscope, we applied STARCAM to a variety of decimeter-scale objects, with applications ranging from cultural heritage to industrial inspection.

Kanghyun Kim, Pavan Chandra Konda, Colin L. Cooke et al.

Standard microscopes offer a variety of settings to help improve the visibility of different specimens to the end microscope user. Increasingly, however, digital microscopes are used to capture images for automated interpretation by computer algorithms (e.g., for feature classification, detection or segmentation), often without any human involvement. In this work, we investigate an approach to jointly optimize multiple microscope settings, together with a classification network, for improved performance with such automated tasks. We explore the interplay between optimization of programmable illumination and pupil transmission, using experimentally imaged blood smears for automated malaria parasite detection, to show that multi-element "learned sensing" outperforms its single-element counterpart. While not necessarily ideal for human interpretation, the network's resulting low-resolution microscope images (20X-comparable) offer a machine learning network sufficient contrast to match the classification performance of corresponding high-resolution imagery (100X-comparable), pointing a path towards accurate automation over large fields-of-view.

Ron Appel, Xavier Burgos-Artizzu, Pietro Perona

We present a simple unified framework for multi-class cost-sensitive boosting. The minimum-risk class is estimated directly, rather than via an approximation of the posterior distribution. Our method jointly optimizes binary weak learners and their corresponding output vectors, requiring classes to share features at each iteration. By training in a cost-sensitive manner, weak learners are invested in separating classes whose discrimination is important, at the expense of less relevant classification boundaries. Additional contributions are a family of loss functions along with proof that our algorithm is Boostable in the theoretical sense, as well as an efficient procedure for growing decision trees for use as weak learners. We evaluate our method on a variety of datasets: a collection of synthetic planar data, common UCI datasets, MNIST digits, SUN scenes, and CUB-200 birds. Results show state-of-the-art performance across all datasets against several strong baselines, including non-boosting multi-class approaches.