Stéphane Mottelet

Cédric Join, Jean Bernier, Stéphane Mottelet et al.

Due to severe mathematical modeling and calibration difficulties open-loop feedforward control is mainly employed today for wastewater denitrification, which is a key ecological issue. In order to improve the resulting poor performances a new model-free control setting and its corresponding "intelligent" controller are introduced. The pitfall of regulating two output variables via a single input variable is overcome by introducing also an open-loop knowledge-based control deduced from the plant behavior. Several convincing computer simulations are presented and discussed.

Stéphane Mottelet, Gil Gaullier, Georges Sadaka

Comprehension of metabolic pathways is considerably enhanced by metabolic flux analysis (MFA-ILE) in isotope labeling experiments. The balance equations are given by hundreds of algebraic (stationary MFA) or ordinary differential equations (nonstationary MFA), and reducing the number of operations is therefore a crucial part of reducing the computation cost. The main bottleneck for deterministic algorithms is the computation of derivatives, particularly for nonstationary MFA. In this article we explain how the overall identification process may be speeded up by using the adjoint approach to compute the gradient of the residual sum of squares. The proposed approach shows significant improvements in terms of complexity and computation time when it is compared with the usual (direct) approach. Numerical results are obtained for the central metabolic pathways of Escherichia coli and are validated against reference software in the stationary case. The methods and algorithms described in this paper are included in the sysmetab software package distributed under an Open Source license at http://forge.scilab.org/index.php/p/sysmetab/.

Vincent Rocher, Cédric Join, Stéphane Mottelet et al.

The recent popularity of post-denitrification processes in the greater Paris area wastewater treatment plants has caused a resurgence of the presence of nitrite in the Seine river. Controlling the production of nitrite during the post-denitrification has thus become a major technical issue. Research studies have been led in the MOCOPEE program (www.mocopee.com) to better understand the underlying mechanisms behind the production of nitrite during wastewater denitrification and to develop technical tools (measurement and control solutions) to assist on-site reductions of nitrite productions. Prior studies have shown that typical methanol dosage strategies produce a varying carbon-to-nitrogen ratio in the reactor, which in turn leads to unstable nitrite concentrations in the effluent. The possibility of adding a model-free control to the actual classical dosage strategy has thus been tested on the SimBio model, which simulates the behavior of wastewater biofilters. The corresponding "intelligent" feedback loop, which is using effluent nitrite concentrations, compensates the classical strategy only when needed. Simulation results show a clear improvement in average nitrite concentration level and level stability in the effluent, without a notable overcost in methanol.