Zongcai Tan

Zongcai Tan, Lan Wei, Dandan Zhang

Optical tweezers (OT) provide piconewton-scale manipulation for delicate biomedical tasks, where visuo-haptic feedback can improve operator awareness by conveying interaction-force cues and trap-stability information. However, visuo-haptic teleoperation frameworks for complex-shaped optical microrobots remain underdeveloped, particularly in multi-trap manipulation scenarios. This paper presents a digital twin framework for virtual visuo-haptic teleoperation of complex-shaped OT-driven microrobots. The framework integrates a digital twin environment, image-based pose and depth estimation, microrobot motion simulation, and model-based haptic rendering within a Robot Operating System (ROS)-connected bimanual teleoperation system. For force modeling, we combine a Multi-Sphere Distributed Manipulation (MSDM) model with optical-force estimation from the Optical Tweezers Toolbox, enabling simulator-driven visuo-haptic feedback. The framework reproduces representative microrobot motion trends and provides haptic force rendering that is numerically consistent with the fitted optical-force model. In simulated cell-delivery tasks, haptic feedback reduced the standard deviations of the contact-force metric and the microrobot-to-trap-center distance metric by 53.2% and 55.2%, respectively, and improved task success from 30% to 80%. These results demonstrate the framework's effectiveness for evaluating visuo-haptic teleoperation strategies for complex-shaped optical microrobots.

Zongcai Tan, Zhenhai Gao

Currently, low-resolution image recognition is confronted with a significant challenge in the field of intelligent traffic perception. Compared to high-resolution images, low-resolution images suffer from small size, low quality, and lack of detail, leading to a notable decrease in the accuracy of traditional neural network recognition algorithms. The key to low-resolution image recognition lies in effective feature extraction. Therefore, this paper delves into the fundamental dimensions of residual modules and their impact on feature extraction and computational efficiency. Based on experiments, we introduce a dual-branch residual network structure that leverages the basic architecture of residual networks and a common feature subspace algorithm. Additionally, it incorporates the utilization of intermediate-layer features to enhance the accuracy of low-resolution image recognition. Furthermore, we employ knowledge distillation to reduce network parameters and computational overhead. Experimental results validate the effectiveness of this algorithm for low-resolution image recognition in traffic environments.

Lan Wei, Zongcai Tan, Kangyi Lu et al.

Optical microrobots actuated by optical tweezers (OT) are important for cell manipulation and microscale assembly, but their autonomous operation depends on accurate 3D perception. Developing such perception systems is challenging because large-scale, high-quality microscopy datasets are scarce, owing to complex fabrication processes and labor-intensive annotation. Although generative AI offers a promising route for data augmentation, existing generative adversarial network (GAN)-based methods struggle to reproduce key optical characteristics, particularly depth-dependent diffraction and defocus effects. To address this limitation, we propose Du-FreqNet, a dual-control, frequency-aware diffusion model for physically consistent microscopy image synthesis. The framework features two independent ControlNet branches to encode microrobot 3D point clouds and depth-specific mesh layers, respectively. We introduce an adaptive frequency-domain loss that dynamically reweights high- and low-frequency components based on the distance to the focal plane. By leveraging differentiable FFT-based supervision, Du-FreqNet captures physically meaningful frequency distributions often missed by pixel-space methods. Trained on a limited dataset (e.g., 80 images per pose), our model achieves controllable, depth-dependent image synthesis, improving SSIM by 20.7% over baselines. Extensive experiments demonstrate that Du-FreqNet generalizes effectively to unseen poses and significantly enhances downstream tasks, including 3D pose and depth estimation, thereby facilitating robust closed-loop control in microrobotic systems.

Kangyi Lu, Lan Wei, Zongcai Tan et al.

Microscale manipulation has advanced substantially in controlled locomotion and targeted transport, yet many biomedical applications require precise and adaptive interaction with biological micro-objects. At these scales, manipulation is realized through three main classes of platforms: embodied microrobots that physically interact as mobile agents, field-mediated systems that generate contactless trapping or manipulation forces, and externally actuated end-effectors that interact through remotely driven physical tools. Unlike macroscale manipulators, these systems function in fluidic, confined, and surface-dominated environments characterized by negligible inertia, dominant interfacial forces, and soft, heterogeneous, and fragile targets. Consequently, classical assumptions of dexterous manipulation, including rigid-body contact, stable grasping, and rich proprioceptive feedback, become difficult to maintain. This review introduces micro-dexterity as a framework for analyzing biological micromanipulation through the coupled roles of embodiment, perception, and control. We examine how classical manipulation primitives, including pushing, reorientation, grasping, and cooperative manipulation, are reformulated at the microscale; compare the architectures that enable them, from contact-based micromanipulators to contactless field-mediated systems and cooperative multi-agent platforms; and review the perception and control strategies required for task execution. We identify the current dexterity gap between laboratory demonstrations and clinically relevant biological manipulation, and outline key challenges for future translation.

Zongcai Tan, Lan Wei, Dandan Zhang

Precise pose estimation of optical microrobots is essential for enabling high-precision object tracking and autonomous biological studies. However, current methods rely heavily on large, high-quality microscope image datasets, which are difficult and costly to acquire due to the complexity of microrobot fabrication and the labour-intensive labelling. Digital twin systems offer a promising path for sim-to-real data augmentation, yet existing techniques struggle to replicate complex optical microscopy phenomena, such as diffraction artifacts and depth-dependent imaging.This work proposes a novel physics-informed deep generative learning framework that, for the first time, integrates wave optics-based physical rendering and depth alignment into a generative adversarial network (GAN), to synthesise high-fidelity microscope images for microrobot pose estimation efficiently. Our method improves the structural similarity index (SSIM) by 35.6% compared to purely AI-driven methods, while maintaining real-time rendering speeds (0.022 s/frame).The pose estimator (CNN backbone) trained on our synthetic data achieves 93.9%/91.9% (pitch/roll) accuracy, just 5.0%/5.4% (pitch/roll) below that of an estimator trained exclusively on real data. Furthermore, our framework generalises to unseen poses, enabling data augmentation and robust pose estimation for novel microrobot configurations without additional training data.

Zongcai Tan, Dandan Zhang

Optical tweezers (OT) offer unparalleled capabilities for micromanipulation with submicron precision in biomedical applications. However, controlling conventional multi-trap OT to achieve cooperative manipulation of multiple complex-shaped microrobots in dynamic environments poses a significant challenge. To address this, we introduce Interactive OT Gym, a reinforcement learning (RL)-based simulation platform designed for OT-driven microrobotics. Our platform supports complex physical field simulations and integrates haptic feedback interfaces, RL modules, and context-aware shared control strategies tailored for OT-driven microrobot in cooperative biological object manipulation tasks. This integration allows for an adaptive blend of manual and autonomous control, enabling seamless transitions between human input and autonomous operation. We evaluated the effectiveness of our platform using a cell manipulation task. Experimental results show that our shared control system significantly improves micromanipulation performance, reducing task completion time by approximately 67% compared to using pure human or RL control alone and achieving a 100% success rate. With its high fidelity, interactivity, low cost, and high-speed simulation capabilities, Interactive OT Gym serves as a user-friendly training and testing environment for the development of advanced interactive OT-driven micromanipulation systems and control algorithms. For more details on the project, please see our website https://sites.google.com/view/otgym