Miloš Judaš

Andrija Štajduhar, Tomislav Lipić, Goran Sedmak et al.

In this paper, we present a novel method for analysis and segmentation of laminar structure of the cortex based on tissue characteristics whose change across the gray matter underlies distinctive between cortical layers. We develop and analyze features of individual neurons to investigate changes in cytoarchitectonic differentiation and present a novel high-performance, automated framework for neuron-level histological image analysis. Local tissue and cell descriptors such as density, neuron size and other measures are used for development of more complex neuron features used in machine learning model trained on data manually labeled by three human experts. Final neuron layer classifications were obtained by training a separate model for each expert and combining their probability outputs. Importances of developed neuron features on both global model level and individual prediction level are presented and discussed.

Andrija Štajduhar, Domagoj Džaja, Miloš Judaš et al.

In this paper, we present a novel use of an anisotropic diffusion model for automatic detection of neurons in histological sections of the adult human brain cortex. We use a partial differential equation model to process high resolution images to acquire locations of neuronal bodies. We also present a novel approach in model training and evaluation that considers variability among the human experts, addressing the issue of existence and correctness of the golden standard for neuron and cell counting, used in most of relevant papers. Our method, trained on dataset manually labeled by three experts, has correctly distinguished over 95% of neuron bodies in test data, doing so in time much shorter than other comparable methods.