Thomas F. Burks

Rishik Aggarwal, Krisha Joshi, Pappu Kumar Yadav et al.

Hyperspectral imaging (HSI) allows researchers to study plant traits non-destructively. By capturing hundreds of narrow spectral bands per pixel, it reveals details about plant biochemistry and stress that standard cameras miss. However, processing this data is often challenging. Many labs still rely on loosely organized collections of lab-specific MATLAB or Python scripts, which makes workflows difficult to share and results difficult to reproduce. MVOS_HSI is an open-source Python library that provides an end-to-end workflow for processing leaf-level HSI data. The software handles everything from calibrating raw ENVI files to detecting and clipping individual leaves based on multiple vegetation indices (NDVI, CIRedEdge and GCI). It also includes tools for data augmentation to create training-time variations for machine learning and utilities to visualize spectral profiles. MVOS_HSI can be used as an importable Python library or run directly from the command line. The code and documentation are available on GitHub. By consolidating these common tasks into a single package, MVOS_HSI helps researchers produce consistent and reproducible results in plant phenotyping

Pappu Kumar Yadav, Rishik Aggarwal, Supriya Paudel et al.

Sudden Death Syndrome (SDS), caused by Fusarium virguliforme, poses a significant threat to soybean production. This study presents an AI-driven web application for early detection of SDS on soybean leaves using hyperspectral imaging, enabling diagnosis prior to visible symptom onset. Leaf samples from healthy and inoculated plants were scanned using a portable hyperspectral imaging system (398-1011 nm), and a Genetic Algorithm was employed to select five informative wavelengths (505.4, 563.7, 712.2, 812.9, and 908.4 nm) critical for discriminating infection status. These selected bands were fed into a lightweight Convolutional Neural Network (CNN) to extract spatial-spectral features, which were subsequently classified using ten classical machine learning models. Ensemble classifiers (Random Forest, AdaBoost), Linear SVM, and Neural Net achieved the highest accuracy (>98%) and minimal error across all folds, as confirmed by confusion matrices and cross-validation metrics. Poor performance by Gaussian Process and QDA highlighted their unsuitability for this dataset. The trained models were deployed within a web application that enables users to upload hyperspectral leaf images, visualize spectral profiles, and receive real-time classification results. This system supports rapid and accessible plant disease diagnostics, contributing to precision agriculture practices. Future work will expand the training dataset to encompass diverse genotypes, field conditions, and disease stages, and will extend the system for multiclass disease classification and broader crop applicability.