Pengyao Xie

Ruiming Du, Zhihong Ma, Pengyao Xie et al.

Segmentation of plant point clouds to obtain high-precise morphological traits is essential for plant phenotyping. Although the fast development of deep learning has boosted much research on segmentation of plant point clouds, previous studies mainly focus on the hard voxelization-based or down-sampling-based methods, which are limited to segmenting simple plant organs. Segmentation of complex plant point clouds with a high spatial resolution still remains challenging. In this study, we proposed a deep learning network plant segmentation transformer (PST) to achieve the semantic and instance segmentation of rapeseed plants point clouds acquired by handheld laser scanning (HLS) with the high spatial resolution, which can characterize the tiny siliques as the main traits targeted. PST is composed of: (i) a dynamic voxel feature encoder (DVFE) to aggregate the point features with the raw spatial resolution; (ii) the dual window sets attention blocks to capture the contextual information; and (iii) a dense feature propagation module to obtain the final dense point feature map. The results proved that PST and PST-PointGroup (PG) achieved superior performance in semantic and instance segmentation tasks. For the semantic segmentation, the mean IoU, mean Precision, mean Recall, mean F1-score, and overall accuracy of PST were 93.96%, 97.29%, 96.52%, 96.88%, and 97.07%, achieving an improvement of 7.62%, 3.28%, 4.8%, 4.25%, and 3.88% compared to the second-best state-of-the-art network PAConv. For instance segmentation, PST-PG reached 89.51%, 89.85%, 88.83% and 82.53% in mCov, mWCov, mPerc90, and mRec90, achieving an improvement of 2.93%, 2.21%, 1.99%, and 5.9% compared to the original PG. This study proves that the deep-learning-based point cloud segmentation method has a great potential for resolving dense plant point clouds with complex morphological traits.

Xin Yang, Xuqi Lu, Pengyao Xie et al.

The rice panicle traits significantly influence grain yield, making them a primary target for rice phenotyping studies. However, most existing techniques are limited to controlled indoor environments and difficult to capture the rice panicle traits under natural growth conditions. Here, we developed PanicleNeRF, a novel method that enables high-precision and low-cost reconstruction of rice panicle three-dimensional (3D) models in the field using smartphone. The proposed method combined the large model Segment Anything Model (SAM) and the small model You Only Look Once version 8 (YOLOv8) to achieve high-precision segmentation of rice panicle images. The NeRF technique was then employed for 3D reconstruction using the images with 2D segmentation. Finally, the resulting point clouds are processed to successfully extract panicle traits. The results show that PanicleNeRF effectively addressed the 2D image segmentation task, achieving a mean F1 Score of 86.9% and a mean Intersection over Union (IoU) of 79.8%, with nearly double the boundary overlap (BO) performance compared to YOLOv8. As for point cloud quality, PanicleNeRF significantly outperformed traditional SfM-MVS (structure-from-motion and multi-view stereo) methods, such as COLMAP and Metashape. The panicle length was then accurately extracted with the rRMSE of 2.94% for indica and 1.75% for japonica rice. The panicle volume estimated from 3D point clouds strongly correlated with the grain number (R2 = 0.85 for indica and 0.82 for japonica) and grain mass (0.80 for indica and 0.76 for japonica). This method provides a low-cost solution for high-throughput in-field phenotyping of rice panicles, accelerating the efficiency of rice breeding.

Xin Yang, Ruiming Du, Hanyang Huang et al.

Organ segmentation of plant point clouds is a prerequisite for the high-resolution and accurate extraction of organ-level phenotypic traits. Although the fast development of deep learning has boosted much research on segmentation of plant point clouds, the existing techniques for organ segmentation still face limitations in resolution, segmentation accuracy, and generalizability across various plant species. In this study, we proposed a novel approach called plant segmentation neural radiance fields (PlantSegNeRF), aiming to directly generate high-precision instance point clouds from multi-view RGB image sequences for a wide range of plant species. PlantSegNeRF performed 2D instance segmentation on the multi-view images to generate instance masks for each organ with a corresponding ID. The multi-view instance IDs corresponding to the same plant organ were then matched and refined using a specially designed instance matching module. The instance NeRF was developed to render an implicit scene, containing color, density, semantic and instance information. The implicit scene was ultimately converted into high-precision plant instance point clouds based on the volume density. The results proved that in semantic segmentation of point clouds, PlantSegNeRF outperformed the commonly used methods, demonstrating an average improvement of 16.1%, 18.3%, 17.8%, and 24.2% in precision, recall, F1-score, and IoU compared to the second-best results on structurally complex species. More importantly, PlantSegNeRF exhibited significant advantages in plant point cloud instance segmentation tasks. Across all plant species, it achieved average improvements of 11.7%, 38.2%, 32.2% and 25.3% in mPrec, mRec, mCov, mWCov, respectively. This study extends the organ-level plant phenotyping and provides a high-throughput way to supply high-quality 3D data for the development of large-scale models in plant science.

Pengyao Xie, Zhihong Ma, Ruiming Du et al.

Fusion of 3D and MS imaging data has a great potential for high-throughput plant phenotyping of structural and biochemical as well as physiological traits simultaneously, which is important for decision support in agriculture and for crop breeders in selecting the best genotypes. However, lacking of 3D data integrity of various plant canopy structures and low-quality of MS images caused by the complex illumination effects make a great challenge, especially at the proximal imaging scale. Therefore, this study proposed a novel approach for adaptive data acquisition and radiometric calibration to generate high-quality 3DMPCs of plants. An efficient NBV planning method based on an UGV plant phenotyping system with a multi-sensor-equipped robotic arm was proposed to achieve adaptive data acquisition. The NeREF was employed to predict the DN values of the hemispherical reference for radiometric calibration. For NBV planning, the average total time for single plant at a joint speed of 1.55 rad/s was about 62.8 s, with an average reduction of 18.0% compared to the unplanned. The integrity of the whole-plant data was improved by an average of 23.6% compared to the fixed viewpoints alone. Compared with the ASD measurements, the RMSE of the reflectance spectra obtained from 3DMPCs at different regions of interest was 0.08 with an average decrease of 58.93% compared to the results obtained from the single-frame of MS images without 3D radiometric calibration. The 3D-calibrated plant 3DMPCs improved the predictive accuracy of PLSR for chlorophyll content, with an average increase of 0.07 in R2 and an average decrease of 21.25% in RMSE. Our approach introduced a fresh perspective on generating high-quality 3DMPCs of plants under the natural light condition, enabling more precise analysis of plant morphological and physiological parameters.