Eirini Troullinou

Eirini Troullinou, Grigorios Tsagkatakis, Spyridon Chavlis et al.

Identification of different neuronal cell types is critical for understanding their contribution to brain functions. Yet, automated and reliable classification of neurons remains a challenge, primarily because of their biological complexity. Typical approaches include laborious and expensive immunohistochemical analysis while feature extraction algorithms based on cellular characteristics have recently been proposed. The former rely on molecular markers, which are often expressed in many cell types, while the latter suffer from similar issues: finding features that are distinctive for each class has proven to be equally challenging. Moreover, both approaches are time consuming and demand a lot of human intervention. In this work we establish the first, automated cell-type classification method that relies on neuronal activity rather than molecular or cellular features. We test our method on a real-world dataset comprising of raw calcium activity signals for four neuronal types. We compare the performance of three different deep learning models and demonstrate that our method can achieve automated classification of neuronal cell types with unprecedented accuracy.

Eirini Troullinou, Grigorios Tsagkatakis, Ganna Palagina et al.

The field of neuroscience is experiencing rapid growth in the complexity and quantity of the recorded neural activity allowing us unprecedented access to its dynamics in different brain areas. The objective of this work is to discover directly from the experimental data rich and comprehensible models for brain function that will be concurrently robust to noise. Considering this task from the perspective of dimensionality reduction, we develop an innovative, robust to noise dictionary learning framework based on adversarial training methods for the identification of patterns of synchronous firing activity as well as within a time lag. We employ real-world binary datasets describing the spontaneous neuronal activity of laboratory mice over time, and we aim to their efficient low-dimensional representation. The results on the classification accuracy for the discrimination between the clean and the adversarial-noisy activation patterns obtained by an SVM classifier highlight the efficacy of the proposed scheme compared to other methods, and the visualization of the dictionary's distribution demonstrates the multifarious information that we obtain from it.