Arno Onken

Bryan M. Li, Isabel M. Cornacchia, Nathalie L. Rochefort et al.

Accurate predictive models of the visual cortex neural response to natural visual stimuli remain a challenge in computational neuroscience. In this work, we introduce V1T, a novel Vision Transformer based architecture that learns a shared visual and behavioral representation across animals. We evaluate our model on two large datasets recorded from mouse primary visual cortex and outperform previous convolution-based models by more than 12.7% in prediction performance. Moreover, we show that the self-attention weights learned by the Transformer correlate with the population receptive fields. Our model thus sets a new benchmark for neural response prediction and can be used jointly with behavioral and neural recordings to reveal meaningful characteristic features of the visual cortex.

Eddie L. Ungless, Nikolas Vitsakis, Zeerak Talat et al.

This whitepaper offers an overview of the ethical considerations surrounding research into or with large language models (LLMs). As LLMs become more integrated into widely used applications, their societal impact increases, bringing important ethical questions to the forefront. With a growing body of work examining the ethical development, deployment, and use of LLMs, this whitepaper provides a comprehensive and practical guide to best practices, designed to help those in research and in industry to uphold the highest ethical standards in their work.

Eddie L. Ungless, Nikolas Vitsakis, Zeerak Talat et al.

There is a significant body of work looking at the ethical considerations of large language models (LLMs): critiquing tools to measure performance and harms; proposing toolkits to aid in ideation; discussing the risks to workers; considering legislation around privacy and security etc. As yet there is no work that integrates these resources into a single practical guide that focuses on LLMs; we attempt this ambitious goal. We introduce 'LLM Ethics Whitepaper', which we provide as an open and living resource for NLP practitioners, and those tasked with evaluating the ethical implications of others' work. Our goal is to translate ethics literature into concrete recommendations and provocations for thinking with clear first steps, aimed at computer scientists. 'LLM Ethics Whitepaper' distils a thorough literature review into clear Do's and Don'ts, which we present also in this paper. We likewise identify useful toolkits to support ethical work. We refer the interested reader to the full LLM Ethics Whitepaper, which provides a succinct discussion of ethical considerations at each stage in a project lifecycle, as well as citations for the hundreds of papers from which we drew our recommendations. The present paper can be thought of as a pocket guide to conducting ethical research with LLMs.

Salvatore Esposito, Daniel Rebain, Arno Onken et al.

Accurate segmentation of vascular networks from sparse CT scan slices remains a significant challenge in medical imaging, particularly due to the thin, branching nature of vessels and the inherent sparsity between imaging planes. Existing deep learning approaches, based on binary voxel classification, often struggle with structural continuity and geometric fidelity. To address this challenge, we present VesselSDF, a novel framework that leverages signed distance fields (SDFs) for robust vessel reconstruction. Our method reformulates vessel segmentation as a continuous SDF regression problem, where each point in the volume is represented by its signed distance to the nearest vessel surface. This continuous representation inherently captures the smooth, tubular geometry of blood vessels and their branching patterns. We obtain accurate vessel reconstructions while eliminating common SDF artifacts such as floating segments, thanks to our adaptive Gaussian regularizer which ensures smoothness in regions far from vessel surfaces while producing precise geometry near the surface boundaries. Our experimental results demonstrate that VesselSDF significantly outperforms existing methods and preserves vessel geometry and connectivity, enabling more reliable vascular analysis in clinical settings.

Thomas Walker, Salvatore Esposito, Daniel Rebain et al.

Reconstructing complex structures from planar cross-sections is a challenging problem, with wide-reaching applications in medical imaging, manufacturing, and topography. Out-of-the-box point cloud reconstruction methods can often fail due to the data sparsity between slicing planes, while current bespoke methods struggle to reconstruct thin geometric structures and preserve topological continuity. This is important for medical applications where thin vessel structures are present in CT and MRI scans. This paper introduces CrossSDF, a novel approach for extracting a 3D signed distance field from 2D signed distances generated from planar contours. Our approach makes the training of neural SDFs contour-aware by using losses designed for the case where geometry is known within 2D slices. Our results demonstrate a significant improvement over existing methods, effectively reconstructing thin structures and producing accurate 3D models without the interpolation artifacts or over-smoothing of prior approaches.

Salvatore Esposito, Qingshan Xu, Kacper Kania et al.

We introduce a new generative approach for synthesizing 3D geometry and images from single-view collections. Most existing approaches predict volumetric density to render multi-view consistent images. By employing volumetric rendering using neural radiance fields, they inherit a key limitation: the generated geometry is noisy and unconstrained, limiting the quality and utility of the output meshes. To address this issue, we propose GeoGen, a new SDF-based 3D generative model trained in an end-to-end manner. Initially, we reinterpret the volumetric density as a Signed Distance Function (SDF). This allows us to introduce useful priors to generate valid meshes. However, those priors prevent the generative model from learning details, limiting the applicability of the method to real-world scenarios. To alleviate that problem, we make the transformation learnable and constrain the rendered depth map to be consistent with the zero-level set of the SDF. Through the lens of adversarial training, we encourage the network to produce higher fidelity details on the output meshes. For evaluation, we introduce a synthetic dataset of human avatars captured from 360-degree camera angles, to overcome the challenges presented by real-world datasets, which often lack 3D consistency and do not cover all camera angles. Our experiments on multiple datasets show that GeoGen produces visually and quantitatively better geometry than the previous generative models based on neural radiance fields.

Bryan M. Li, Theoklitos Amvrosiadis, Nathalie Rochefort et al.

Understanding how activity in neural circuits reshapes following task learning could reveal fundamental mechanisms of learning. Thanks to the recent advances in neural imaging technologies, high-quality recordings can be obtained from hundreds of neurons over multiple days or even weeks. However, the complexity and dimensionality of population responses pose significant challenges for analysis. Existing methods of studying neuronal adaptation and learning often impose strong assumptions on the data or model, resulting in biased descriptions that do not generalize. In this work, we use a variant of deep generative models called - CycleGAN, to learn the unknown mapping between pre- and post-learning neural activities recorded $\textit{in vivo}$. We develop an end-to-end pipeline to preprocess, train and evaluate calcium fluorescence signals, and a procedure to interpret the resulting deep learning models. To assess the validity of our method, we first test our framework on a synthetic dataset with known ground-truth transformation. Subsequently, we applied our method to neural activities recorded from the primary visual cortex of behaving mice, where the mice transition from novice to expert-level performance in a visual-based virtual reality experiment. We evaluate model performance on generated calcium signals and their inferred spike trains. To maximize performance, we derive a novel approach to pre-sort neurons such that convolutional-based networks can take advantage of the spatial information that exists in neural activities. In addition, we incorporate visual explanation methods to improve the interpretability of our work and gain insights into the learning process as manifested in the cellular activities. Together, our results demonstrate that analyzing neuronal learning processes with data-driven deep unsupervised methods holds the potential to unravel changes in an unbiased way.

Cole Hurwitz, Nina Kudryashova, Arno Onken et al.

Modern recording technologies now enable simultaneous recording from large numbers of neurons. This has driven the development of new statistical models for analyzing and interpreting neural population activity. Here we provide a broad overview of recent developments in this area. We compare and contrast different approaches, highlight strengths and limitations, and discuss biological and mechanistic insights that these methods provide.

Bryan M. Li, Theoklitos Amvrosiadis, Nathalie Rochefort et al.

Calcium imaging has become a powerful and popular technique to monitor the activity of large populations of neurons in vivo. However, for ethical considerations and despite recent technical developments, recordings are still constrained to a limited number of trials and animals. This limits the amount of data available from individual experiments and hinders the development of analysis techniques and models for more realistic sizes of neuronal populations. The ability to artificially synthesize realistic neuronal calcium signals could greatly alleviate this problem by scaling up the number of trials. Here, we propose a Generative Adversarial Network (GAN) model to generate realistic calcium signals as seen in neuronal somata with calcium imaging. To this end, we propose CalciumGAN, a model based on the WaveGAN architecture and train it on calcium fluorescent signals with the Wasserstein distance. We test the model on artificial data with known ground-truth and show that the distribution of the generated signals closely resembles the underlying data distribution. Then, we train the model on real calcium traces recorded from the primary visual cortex of behaving mice and confirm that the deconvolved spike trains match the statistics of the recorded data. Together, these results demonstrate that our model can successfully generate realistic calcium traces, thereby providing the means to augment existing datasets of neuronal activity for enhanced data exploration and modelling.

Nina Kudryashova, Theoklitos Amvrosiadis, Nathalie Dupuy et al.

One of the main challenges in current systems neuroscience is the analysis of high-dimensional neuronal and behavioral data that are characterized by different statistics and timescales of the recorded variables. We propose a parametric copula model which separates the statistics of the individual variables from their dependence structure, and escapes the curse of dimensionality by using vine copula constructions. We use a Bayesian framework with Gaussian Process (GP) priors over copula parameters, conditioned on a continuous task-related variable. We validate the model on synthetic data and compare its performance in estimating mutual information against the commonly used non-parametric algorithms. Our model provides accurate information estimates when the dependencies in the data match the parametric copulas used in our framework. When the exact density estimation with a parametric model is not possible, our Copula-GP model is still able to provide reasonable information estimates, close to the ground truth and comparable to those obtained with a neural network estimator. Finally, we apply our framework to real neuronal and behavioral recordings obtained in awake mice. We demonstrate the ability of our framework to 1) produce accurate and interpretable bivariate models for the analysis of inter-neuronal noise correlations or behavioral modulations; 2) expand to more than 100 dimensions and measure information content in the whole-population statistics. These results demonstrate that the Copula-GP framework is particularly useful for the analysis of complex multidimensional relationships between neuronal, sensory and behavioral data.

Bennet Breier, Arno Onken

Semmelhack et al. (2014) have achieved high classification accuracy in distinguishing swim bouts of zebrafish using a Support Vector Machine (SVM). Convolutional Neural Networks (CNNs) have reached superior performance in various image recognition tasks over SVMs, but these powerful networks remain a black box. Reaching better transparency helps to build trust in their classifications and makes learned features interpretable to experts. Using a recently developed technique called Deep Taylor Decomposition, we generated heatmaps to highlight input regions of high relevance for predictions. We find that our CNN makes predictions by analyzing the steadiness of the tail's trunk, which markedly differs from the manually extracted features used by Semmelhack et al. (2014). We further uncovered that the network paid attention to experimental artifacts. Removing these artifacts ensured the validity of predictions. After correction, our best CNN beats the SVM by 6.12%, achieving a classification accuracy of 96.32%. Our work thus demonstrates the utility of AI explainability for CNNs.

Shanshan Jia, Zhaofei Yu, Arno Onken et al.

Neuronal circuits formed in the brain are complex with intricate connection patterns. Such complexity is also observed in the retina as a relatively simple neuronal circuit. A retinal ganglion cell receives excitatory inputs from neurons in previous layers as driving forces to fire spikes. Analytical methods are required that can decipher these components in a systematic manner. Recently a method termed spike-triggered non-negative matrix factorization (STNMF) has been proposed for this purpose. In this study, we extend the scope of the STNMF method. By using the retinal ganglion cell as a model system, we show that STNMF can detect various computational properties of upstream bipolar cells, including spatial receptive field, temporal filter, and transfer nonlinearity. In addition, we recover synaptic connection strengths from the weight matrix of STNMF. Furthermore, we show that STNMF can separate spikes of a ganglion cell into a few subsets of spikes where each subset is contributed by one presynaptic bipolar cell. Taken together, these results corroborate that STNMF is a useful method for deciphering the structure of neuronal circuits.

Manuel Molano-Mazon, Arno Onken, Eugenio Piasini et al.

The ability to synthesize realistic patterns of neural activity is crucial for studying neural information processing. Here we used the Generative Adversarial Networks (GANs) framework to simulate the concerted activity of a population of neurons. We adapted the Wasserstein-GAN variant to facilitate the generation of unconstrained neural population activity patterns while still benefiting from parameter sharing in the temporal domain. We demonstrate that our proposed GAN, which we termed Spike-GAN, generates spike trains that match accurately the first- and second-order statistics of datasets of tens of neurons and also approximates well their higher-order statistics. We applied Spike-GAN to a real dataset recorded from salamander retina and showed that it performs as well as state-of-the-art approaches based on the maximum entropy and the dichotomized Gaussian frameworks. Importantly, Spike-GAN does not require to specify a priori the statistics to be matched by the model, and so constitutes a more flexible method than these alternative approaches. Finally, we show how to exploit a trained Spike-GAN to construct 'importance maps' to detect the most relevant statistical structures present in a spike train. Spike-GAN provides a powerful, easy-to-use technique for generating realistic spiking neural activity and for describing the most relevant features of the large-scale neural population recordings studied in modern systems neuroscience.