Jean-Christophe Olivo-Marin

Antoine Habis, Vannary Meas-Yedid, Elsa Angelini et al.

This paper introduces a novel approach that combines unsupervised active contour models with deep learning for robust and adaptive image segmentation. Indeed, traditional active contours, provide a flexible framework for contour evolution and learning offers the capacity to learn intricate features and patterns directly from raw data. Our proposed methodology leverages the strengths of both paradigms, presenting a framework for both unsupervised and one-shot approaches for image segmentation. It is capable of capturing complex object boundaries without the need for extensive labeled training data. This is particularly required in histology, a field facing a significant shortage of annotations due to the challenging and time-consuming nature of the annotation process. We illustrate and compare our results to state of the art methods on a histology dataset and show significant improvements.

Carlos Garcia-Lopez-de-Haro, Caterina Fuster-Barcelo, Curtis T. Rueden et al. · cambridge

Mask annotation remains a significant bottleneck in AI-driven biomedical image analysis due to its labor-intensive nature. To address this challenge, we introduce SAMJ, a user-friendly ImageJ/Fiji plugin leveraging the Segment Anything Model (SAM). SAMJ enables seamless, interactive annotations with one-click installation on standard computers. Designed for real-time object delineation in large scientific images, SAMJ is an easy-to-use solution that simplifies and accelerates the creation of labeled image datasets.

Raphael Reme, Alasdair Newson, Elsa Angelini et al.

Accurately tracking neuronal activity in behaving animals presents significant challenges due to complex motions and background noise. The lack of annotated datasets limits the evaluation and improvement of such tracking algorithms. To address this, we developed SINETRA, a versatile simulator that generates synthetic tracking data for particles on a deformable background, closely mimicking live animal recordings. This simulator produces annotated 2D and 3D videos that reflect the intricate movements seen in behaving animals like Hydra Vulgaris. We evaluated four state-of-the-art tracking algorithms highlighting the current limitations of these methods in challenging scenarios and paving the way for improved cell tracking techniques in dynamic biological systems.

Antoine Habis, Roy Rosman Nathanson, Vannary Meas-Yedid et al.

This paper proposes a dynamic interactive and weakly supervised segmentation method with minimal user interactions to address two major challenges in the segmentation of whole slide histopathology images. First, the lack of hand-annotated datasets to train algorithms. Second, the lack of interactive paradigms to enable a dialogue between the pathologist and the machine, which can be a major obstacle for use in clinical routine. We therefore propose a fast and user oriented method to bridge this gap by giving the pathologist control over the final result while limiting the number of interactions needed to achieve a good result (over 90\% on all our metrics with only 4 correction scribbles).

Rituparna Sarkar, Suvadip Mukherjee, Elisabeth Labruyère et al.

Detecting and segmenting individual cells from microscopy images is critical to various life science applications. Traditional cell segmentation tools are often ill-suited for applications in brightfield microscopy due to poor contrast and intensity heterogeneity, and only a small subset are applicable to segment cells in a cluster. In this regard, we introduce a novel supervised technique for cell segmentation in a multi-task learning paradigm. A combination of a multi-task loss, based on the region and cell boundary detection, is employed for an improved prediction efficiency of the network. The learning problem is posed in a novel min-max framework which enables adaptive estimation of the hyper-parameters in an automatic fashion. The region and cell boundary predictions are combined via morphological operations and active contour model to segment individual cells. The proposed methodology is particularly suited to segment touching cells from brightfield microscopy images without manual interventions. Quantitatively, we observe an overall Dice score of 0.93 on the validation set, which is an improvement of over 15.9% on a recent unsupervised method, and outperforms the popular supervised U-net algorithm by at least $5.8\%$ on average.