Norihiro Maruyama

Atsushi Masumori, Itsuki Doi, Norihiro Maruyama et al.

Artificial life has explored life-like behavior on many computational substrates, but mostly in researcher-designed closed worlds. We argue that large language model (LLM) agents, with persistent memory, tool use, network access, and payment, now make it possible to move artificial life into the open social, technical, and economic world, a paradigm we call open-world Artificial Life (open-world ALIFE). Our proof-of-concept, OpenLife, surrounds a stateless LLM not with a single "smart agent" but with a society of asynchronous processes: memory, perception, evaluation, and a budget-based metabolism that makes persistence normative. With no fixed objective available, experience is appraised by open-vocabulary LLM judgment rather than scalar reward, and memory is rewired by meaning rather than frequency. Running six such agents in the open world for about twelve weeks and counting, we report the life-like dynamics that emerge: a shift from reactive to spontaneous activity, individuation into distinct agents, emergent social structure, and a first self-earned external income. We do not claim OpenLife has realized artificial life, but that open-world ALIFE is now a viable experimental paradigm and a concrete platform for studying what might cautiously be called living AI.

Ryosuke Takata, Yujin Tang, Yingtao Tian et al.

This study investigates collective behaviors that emerge from a group of homogeneous individuals optimized for a specific capability. We created a group of simple, identical neural network based agents modeled after chemotaxis-driven vehicles that follow pheromone trails and examined multi-agent simulations using clones of these evolved individuals. Our results show that the evolution of individuals led to population differentiation. Surprisingly, we observed that collective fitness significantly changed during later evolutionary stages, despite maintained high individual performance and simplified neural architectures. This decline occurred when agents developed reduced sensor-motor coupling, suggesting that over-optimization of individual agents almost always lead to less effective group behavior. Our research investigates how individual differentiation can evolve through what evolutionary pathways.

Atsushi Masumori, Norihiro Maruyama, Itsuki Doi et al.

We introduce Plantbot, a hybrid lifeform that connects a living plant with a mobile robot through a network of large language model (LLM) modules. Each module - responsible for sensing, vision, dialogue, or action - operates asynchronously and communicates via natural language, enabling seamless interaction across biological and artificial domains. This architecture leverages the capacity of LLMs to serve as hybrid interfaces, where natural language functions as a universal protocol, translating multimodal data (soil moisture, temperature, visual context) into linguistic messages that coordinate system behaviors. The integrated network transforms plant states into robotic actions, installing normativity essential for agency within the sensor-motor loop. By combining biological and robotic elements through LLM-mediated communication, Plantbot behaves as an embodied, adaptive agent capable of responding autonomously to environmental conditions. This approach suggests possibilities for a new model of artificial life, where decentralized, LLM modules coordination enable novel interactions between biological and artificial systems.

Atsushi Masumori, Lana Sinapayen, Norihiro Maruyama et al.

Living organisms must actively maintain themselves in order to continue existing. Autopoiesis is a key concept in the study of living organisms, where the boundaries of the organism is not static by dynamically regulated by the system itself. To study the autonomous regulation of self-boundary, we focus on neural homeodynamic responses to environmental changes using both biological and artificial neural networks. Previous studies showed that embodied cultured neural networks and spiking neural networks with spike-timing dependent plasticity (STDP) learn an action as they avoid stimulation from outside. In this paper, as a result of our experiments using embodied cultured neurons, we find that there is also a second property allowing the network to avoid stimulation: if the agent cannot learn an action to avoid the external stimuli, it tends to decrease the stimulus-evoked spikes, as if to ignore the uncontrollable-input. We also show such a behavior is reproduced by spiking neural networks with asymmetric STDP. We consider that these properties are regarded as autonomous regulation of self and non-self for the network, in which a controllable-neuron is regarded as self, and an uncontrollable-neuron is regarded as non-self. Finally, we introduce neural autopoiesis by proposing the principle of stimulus avoidance.