Honghan Li

Nhi Nguyen, Le Nguyen, Honghan Li et al.

Video-based remote photoplethysmography (rPPG) has emerged as a promising technology for non-contact vital sign monitoring, especially under controlled conditions. However, the accurate measurement of vital signs in real-world scenarios faces several challenges, including artifacts induced by videocodecs, low-light noise, degradation, low dynamic range, occlusions, and hardware and network constraints. In this article, we systematically investigate comprehensive investigate these issues, measuring their detrimental effects on the quality of rPPG measurements. Additionally, we propose practical strategies for mitigating these challenges to improve the dependability and resilience of video-based rPPG systems. We detail methods for effective biosignal recovery in the presence of network limitations and present denoising and inpainting techniques aimed at preserving video frame integrity. Through extensive evaluations and direct comparisons, we demonstrate the effectiveness of the approaches in enhancing rPPG measurements under challenging environments, contributing to the development of more reliable and effective remote vital sign monitoring technologies.

Li Honghan, Daiki Matsunaga, Tsubasa S. Matsui et al.

We propose an image-based cellular contractile force evaluation method using a machine learning technique. We use a special substrate that exhibits wrinkles when cells grab the substrate and contract, and the wrinkles can be used to visualize the force magnitude and direction. In order to extract wrinkles from the microscope images, we develop a new CNN (convolutional neural network) architecture SW-UNet (small-world U-Net), which is a CNN that reflects the concept of the small-world network. The SW-UNet shows better performance in wrinkle segmentation task compared to other methods: the error (Euclidean distance) of SW-UNet is 4.9 times smaller than 2D-FFT (fast Fourier transform) based segmentation approach, and is 2.9 times smaller than U-Net. As a demonstration, we compare the contractile force of U2OS (human osteosarcoma) cells and show that cells with a mutation in the KRAS oncogne show larger force compared to the wild-type cells. Our new machine learning based algorithm provides us an efficient, automated and accurate method to evaluate the cell contractile force.