Gregory Kucherov

Karel Brinda, Maciej Sykulski, Gregory Kucherov

Metagenomics is a powerful approach to study genetic content of environmental samples that has been strongly promoted by NGS technologies. To cope with massive data involved in modern metagenomic projects, recent tools [4, 39] rely on the analysis of k-mers shared between the read to be classified and sampled reference genomes. Within this general framework, we show in this work that spaced seeds provide a significant improvement of classification accuracy as opposed to traditional contiguous k-mers. We support this thesis through a series a different computational experiments, including simulations of large-scale metagenomic projects. Scripts and programs used in this study, as well as supplementary material, are available from http://github.com/gregorykucherov/spaced-seeds-for-metagenomics.

Dominik Köppl, Gregory Kucherov

The size of the \textit{smallest suffixient set} of positions of a string recently emerged as a new measure of string \textit{repetitiveness} -- a measure reflecting how much of repetitive content the string contains. We study how to maintain the smallest suffixient set online in near-real-time, that is with small (in our case, polyloglog) worst-case time on processing each letter. Two frameworks are considered: when the text is given letter-by-letter in either a right-to-left or left-to-right direction. Our central algorithmic tool is Weiner's suffix tree algorithm and associated algorithmic primitives for its efficient implementation.