Hongjun Wu

Chenxi Wang, Hongjun Wu, Zhi Jin

Recently, Fourier frequency information has attracted much attention in Low-Light Image Enhancement (LLIE). Some researchers noticed that, in the Fourier space, the lightness degradation mainly exists in the amplitude component and the rest exists in the phase component. By incorporating both the Fourier frequency and the spatial information, these researchers proposed remarkable solutions for LLIE. In this work, we further explore the positive correlation between the magnitude of amplitude and the magnitude of lightness, which can be effectively leveraged to improve the lightness of low-light images in the Fourier space. Moreover, we find that the Fourier transform can extract the global information of the image, and does not introduce massive neural network parameters like Multi-Layer Perceptrons (MLPs) or Transformer. To this end, a two-stage Fourier-based LLIE network (FourLLIE) is proposed. In the first stage, we improve the lightness of low-light images by estimating the amplitude transform map in the Fourier space. In the second stage, we introduce the Signal-to-Noise-Ratio (SNR) map to provide the prior for integrating the global Fourier frequency and the local spatial information, which recovers image details in the spatial space. With this ingenious design, FourLLIE outperforms the existing state-of-the-art (SOTA) LLIE methods on four representative datasets while maintaining good model efficiency.

Li Xiao, Liqing Liu, Hongjun Wu et al.

Recent advances in 2D large-scale and 3D volume electron microscopy have stimulated the rapid development of nanoscale functional analysis at the tissue and organ levels. Digitizing the cell by mapping the intricate organellar networks into its physiological and pathological textures will revolutionarize the contents of cell atlases. To meet the requirements of characterizing intracellular organelles and their interactions within defined cellular cohorts at tissue level, we have developed DeepOrganelle. It adopts a lightweighted Mask2Former frameworks as a universal segmentor and is capable of segmenting and extracting organelles within different cell types, performing statistical quantitative analysis, as well as visualizing and quantifying the spatial distribution of organelle morphologies and interactions across different cell types at tissue scales. Using DeepOrganelle, we systemically perform cross-scale quantification of membrane contact sites(MCSs) dynamics across the progression of the seminiferous epithelial cycle, covering 12 distinct developmental stages and 24 statuses of germ cells. DeepOrganelle uncovers the spatiotemporal gradient of the germ cell differentiation atlas according to different types of organelles and their interactions. Noticeably, it discovers a waved pattern of mitochondria(Mito)-endoplasmic reticulum(ER) contact with a significant increase specifically at Stage X pachytene preceding the transition to diplotene, which aligns well with a newly reported experiment that mitochondrial metabolic proteins like PDHA2 are essential for this transition by maintaining ATP supply for double-strand break(DSB) repair. DeepOrganelle also observes a dynamic restructuring of the blood-testis barrier and stage-specific reorganization of organelle topography in Sertoli cells from preleptotene to leptotene phases of prophase I.