John O'Sullivan

Vuong M. Ngo, Edward Bolger, Stan Goodwin et al.

Exosomes are small vesicles crucial for cell signaling and disease biomarkers. Due to their complexity, an "omics" approach is preferable to individual biomarkers. While Raman spectroscopy is effective for exosome analysis, it requires high sample concentrations and has limited sensitivity to lipids and proteins. Surface-enhanced Raman spectroscopy helps overcome these challenges. In this study, we leverage Neo4j graph databases to organize 3,045 Raman spectra of exosomes, enhancing data generalization. To further refine spectral analysis, we introduce a novel spectral filtering process that integrates the PageRank Filter with optimal Dimensionality Reduction. This method improves feature selection, resulting in superior classification performance. Specifically, the Extra Trees model, using our spectral processing approach, achieves 0.76 and 0.857 accuracy in classifying hyperglycemic, hypoglycemic, and normal exosome samples based on Raman spectra and surface, respectively, with group 10-fold cross-validation. Our results show that graph-based spectral filtering combined with optimal dimensionality reduction significantly improves classification accuracy by reducing noise while preserving key biomarker signals. This novel framework enhances Raman-based exosome analysis, expanding its potential for biomedical applications, disease diagnostics, and biomarker discovery.

Konstantin Läufer, John O'Sullivan, George K. Thiruvathukal

In testing stateful abstractions, it is often necessary to record interactions, such as method invocations, and express assertions over these interactions. Following the Test Spy design pattern, we can reify such interactions programmatically through additional mutable state. Alternatively, a mocking framework, such as Mockito, can automatically generate test spies that allow us to record the interactions and express our expectations in a declarative domain-specific language. According to our study of the test code for Scala's Iterator trait, the latter approach can lead to a significant reduction of test code complexity in terms of metrics such as code size (in some cases over 70% smaller), cyclomatic complexity, and amount of additional mutable state required. In this tools paper, we argue that the resulting test code is not only more maintainable, readable, and intentional, but also a better stylistic match for the Scala community than manually implemented, explicitly stateful test spies.