Sarah E. Jones

Sarah E. Jones, Timilehin Ayanlade, Benjamin Fallen et al.

Soybean production is susceptible to biotic and abiotic stresses, exacerbated by extreme weather events. Water limiting stress, i.e. drought, emerges as a significant risk for soybean production, underscoring the need for advancements in stress monitoring for crop breeding and production. This project combines multi-modal information to identify the most effective and efficient automated methods to investigate drought response. We investigated a set of diverse soybean accessions using multiple sensors in a time series high-throughput phenotyping manner to: (1) develop a pipeline for rapid classification of soybean drought stress symptoms, and (2) investigate methods for early detection of drought stress. We utilized high-throughput time-series phenotyping using UAVs and sensors in conjunction with machine learning (ML) analytics, which offered a swift and efficient means of phenotyping. The red-edge and green bands were most effective to classify canopy wilting stress. The Red-Edge Chlorophyll Vegetation Index (RECI) successfully differentiated susceptible and tolerant soybean accessions prior to visual symptom development. We report pre-visual detection of soybean wilting using a combination of different vegetation indices. These results can contribute to early stress detection methodologies and rapid classification of drought responses in screening nurseries for breeding and production applications.

Koushik Nagasubramanian, Sarah Jones, Asheesh K. Singh et al.

Our overarching goal is to develop an accurate and explainable model for plant disease identification using hyperspectral data. Charcoal rot is a soil borne fungal disease that affects the yield of soybean crops worldwide. Hyperspectral images were captured at 240 different wavelengths in the range of 383 - 1032 nm. We developed a 3D Convolutional Neural Network model for soybean charcoal rot disease identification. Our model has classification accuracy of 95.73\% and an infected class F1 score of 0.87. We infer the trained model using saliency map and visualize the most sensitive pixel locations that enable classification. The sensitivity of individual wavelengths for classification was also determined using the saliency map visualization. We identify the most sensitive wavelength as 733 nm using the saliency map visualization. Since the most sensitive wavelength is in the Near Infrared Region(700 - 1000 nm) of the electromagnetic spectrum, which is also the commonly used spectrum region for determining the vegetation health of the plant, we were more confident in the predictions using our model.

Koushik Nagasubramanian, Sarah Jones, Soumik Sarkar et al.

Charcoal rot is a fungal disease that thrives in warm dry conditions and affects the yield of soybeans and other important agronomic crops worldwide. There is a need for robust, automatic and consistent early detection and quantification of disease symptoms which are important in breeding programs for the development of improved cultivars and in crop production for the implementation of disease control measures for yield protection. Current methods of plant disease phenotyping are predominantly visual and hence are slow and prone to human error and variation. There has been increasing interest in hyperspectral imaging applications for early detection of disease symptoms. However, the high dimensionality of hyperspectral data makes it very important to have an efficient analysis pipeline in place for the identification of disease so that effective crop management decisions can be made. The focus of this work is to determine the minimal number of most effective hyperspectral bands that can distinguish between healthy and diseased specimens early in the growing season. Healthy and diseased hyperspectral data cubes were captured at 3, 6, 9, 12, and 15 days after inoculation. We utilized inoculated and control specimens from 4 different genotypes. Each hyperspectral image was captured at 240 different wavelengths in the range of 383 to 1032 nm. We used a combination of genetic algorithm as an optimizer and support vector machines as a classifier for identification of maximally effective band combinations. A binary classification between healthy and infected samples using six selected band combinations obtained a classification accuracy of 97% and a F1 score of 0.97 for the infected class. The results demonstrated that these carefully chosen bands are more informative than RGB images, and could be used in a multispectral camera for remote identification of charcoal rot infection in soybean.