Bingjie Wang

Razieh Ganjee, Bingjie Wang, Lingyun Wang et al.

Visible light optical coherence tomography (vis-OCT) is gaining traction for retinal imaging due to its high resolution and functional capabilities. However, the significant absorption of hemoglobin in the visible light range leads to pronounced shadow artifacts from retinal blood vessels, posing challenges for accurate layer segmentation. In this study, we present BreakNet, a multi-scale Transformer-based segmentation model designed to address boundary discontinuities caused by these shadow artifacts. BreakNet utilizes hierarchical Transformer and convolutional blocks to extract multi-scale global and local feature maps, capturing essential contextual, textural, and edge characteristics. The model incorporates decoder blocks that expand pathwaproys to enhance the extraction of fine details and semantic information, ensuring precise segmentation. Evaluated on rodent retinal images acquired with prototype vis-OCT, BreakNet demonstrated superior performance over state-of-the-art segmentation models, such as TCCT-BP and U-Net, even when faced with limited-quality ground truth data. Our findings indicate that BreakNet has the potential to significantly improve retinal quantification and analysis.

Xuhui Zhu, Jing Xu, Bingjie Wang et al.

Video Individual Counting (VIC) is a recently introduced task that aims to estimate pedestrian flux from a video. It extends conventional Video Crowd Counting (VCC) beyond the per-frame pedestrian count. In contrast to VCC that only learns to count repeated pedestrian patterns across frames, the key problem of VIC is how to identify co-existent pedestrians between frames, which turns out to be a correspondence problem. Existing VIC approaches, however, mainly follow a one-to-one (O2O) matching strategy where the same pedestrian must be exactly matched between frames, leading to sensitivity to appearance variations or missing detections. In this work, we show that the O2O matching could be relaxed to a one-to-many (O2M) matching problem, which better fits the problem nature of VIC and can leverage the social grouping behavior of walking pedestrians. We therefore introduce OMAN, a simple but effective VIC model with implicit One-to-Many mAtchiNg, featuring an implicit context generator and a one-to-many pairwise matcher. Experiments on the SenseCrowd and CroHD benchmarks show that OMAN achieves the state-of-the-art performance. Code is available at \href{https://github.com/tiny-smart/OMAN}{OMAN}.

Lingyun Wang, Bingjie Wang, Jay Chhablani et al.

Objective: To achieve accurate 3-D reconstruction and quantitative analysis of human retinal vasculature from a single optical coherence tomography angiography (OCTA) scan. Methods: We introduce Freqformer, a novel Transformer-based model featuring a dual-branch architecture that integrates a Transformer layer for capturing global spatial context with a complex-valued frequency-domain module designed for adaptive frequency enhancement. Freqformer was trained using single depth-plane OCTA images, utilizing volumetrically merged OCTA as the ground truth. Performance was evaluated quantitatively through 2-D and 3-D image quality metrics. 2-D networks and their 3-D counterparts were compared to assess the differences between enhancing volume slice by slice and enhancing it by 3-D patches. Furthermore, 3-D quantitative vascular metrics were conducted to quantify human retinal vasculature. Results: Freqformer substantially outperformed existing convolutional neural networks and Transformer-based methods, achieving superior image metrics. Importantly, the enhanced OCTA volumes show strong correlation with the merged volumes on vascular segment count, density, length, and flow index, further underscoring its reliability for quantitative vascular analysis. 3-D counterparts did not yield additional gains in image metrics or downstream 3-D vascular quantification but incurred nearly an order-of-magnitude longer inference time, supporting our 2-D slice-wise enhancement strategy. Additionally, Freqformer showed excellent generalization capability on larger field-of-view scans, surpassing the quality of conventional volumetric merging methods. Conclusion: Freqformer reliably generates high-definition 3-D retinal microvasculature from single-scan OCTA, enabling precise vascular quantification comparable to standard volumetric merging methods.

Shaohua Pi, Razieh Ganjee, Lingyun Wang et al.

This study introduces a groundbreaking optical coherence tomography (OCT) imaging system dedicated for high-throughput screening applications using ex vivo tissue culture. Leveraging OCT's non-invasive, high-resolution capabilities, the system is equipped with a custom-designed motorized platform and tissue detection ability for automated, successive imaging across samples. Transformer-based deep learning segmentation algorithms further ensure robust, consistent, and efficient readouts meeting the standards for screening assays. Validated using retinal explant cultures from a mouse model of retinal degeneration, the system provides robust, rapid, reliable, unbiased, and comprehensive readouts of tissue response to treatments. This fully automated OCT-based system marks a significant advancement in tissue screening, promising to transform drug discovery, as well as other relevant research fields.