Ju Zhang

Mingyang Chen, Luhong Jin, Xuwei Xuan et al.

Live-cell imaging of multiple subcellular structures is essential for understanding subcellular dynamics. However, the conventional multi-color sequential fluorescence microscopy suffers from significant imaging delays and limited number of subcellular structure separate labeling, resulting in substantial limitations for real-time live-cell research applications. Here, we present the Adaptive Explainable Multi-Structure Network (AEMS-Net), a deep-learning framework that enables simultaneous prediction of two subcellular structures from a single image. The model normalizes staining intensity and prioritizes critical image features by integrating attention mechanisms and brightness adaptation layers. Leveraging the Kolmogorov-Arnold representation theorem, our model decomposes learned features into interpretable univariate functions, enhancing the explainability of complex subcellular morphologies. We demonstrate that AEMS-Net allows real-time recording of interactions between mitochondria and microtubules, requiring only half the conventional sequential-channel imaging procedures. Notably, this approach achieves over 30% improvement in imaging quality compared to traditional deep learning methods, establishing a new paradigm for long-term, interpretable live-cell imaging that advances the ability to explore subcellular dynamics.

Ming Li, Peilun Xiao, Ju Zhang

In traditional ELM and its improved versions suffer from the problems of outliers or noises due to overfitting and imbalance due to distribution. We propose a novel hybrid adaptive fuzzy ELM(HA-FELM), which introduces a fuzzy membership function to the traditional ELM method to deal with the above problems. We define the fuzzy membership function not only basing on the distance between each sample and the center of the class but also the density among samples which based on the quantum harmonic oscillator model. The proposed fuzzy membership function overcomes the shortcoming of the traditional fuzzy membership function and could make itself adjusted according to the specific distribution of different samples adaptively. Experiments show the proposed HA-FELM can produce better performance than SVM, ELM, and RELM in text classification.

Ming Li, Peilun Xiao, Ju Zhang

In this paper, we explore SPPIM-based text classification method, and the experiment reveals that the SPPIM method is equal to or even superior than SGNS method in text classification task on three international and standard text datasets, namely 20newsgroups, Reuters52 and WebKB. Comparing to SGNS, although SPPMI provides a better solution, it is not necessarily better than SGNS in text classification tasks. Based on our analysis, SGNS takes into the consideration of weight calculation during decomposition process, so it has better performance than SPPIM in some standard datasets. Inspired by this, we propose a WL-SPPIM semantic model based on SPPIM model, and experiment shows that WL-SPPIM approach has better classification and higher scalability in the text classification task compared with LDA, SGNS and SPPIM approaches.