Gergely Szabó

Gergely Szabó, Paolo Bonaiuti, Andrea Ciliberto et al.

The accurate tracking of live cells using video microscopy recordings remains a challenging task for popular state-of-the-art image processing based object tracking methods. In recent years, several existing and new applications have attempted to integrate deep-learning based frameworks for this task, but most of them still heavily rely on consecutive frame based tracking embedded in their architecture or other premises that hinder generalized learning. To address this issue, we aimed to develop a new deep-learning based tracking method that relies solely on the assumption that cells can be tracked based on their spatio-temporal neighborhood, without restricting it to consecutive frames. The proposed method has the additional benefit that the motion patterns of the cells can be learned completely by the predictor without any prior assumptions, and it has the potential to handle a large number of video frames with heavy artifacts. The efficacy of the proposed method is demonstrated through biologically motivated validation strategies and compared against multiple state-of-the-art cell tracking methods.

Zsófia Molnár, Gergely Szabó, András Horváth

Supervised pretrained models have become widely used in deep learning, especially for image segmentation tasks. However, when applied to specialized datasets such as biomedical imaging, pretrained weights often introduce unintended biases. These biases cause models to assign different levels of importance to different slices, leading to inconsistencies in feature utilization, which can be observed as asymmetries in saliency map distributions. This transfer of color distributions from natural images to non-natural datasets can compromise model performance and reduce the reliability of results. In this study, we investigate the effects of these biases and propose strategies to mitigate them. Through a series of experiments, we test both pretrained and randomly initialized models, comparing their performance and saliency map distributions. Our proposed methods, which aim to neutralize the bias introduced by pretrained color channel weights, demonstrate promising results, offering a practical approach to improving model explainability while maintaining the benefits of pretrained models. This publication presents our findings, providing insights into addressing pretrained weight biases across various deep learning tasks.

Gergely Szabó, Zsófia Molnár, András Horváth

Temporal forward-tracking has been the dominant approach for multi-object segmentation and tracking (MOTS). However, a novel time-symmetric tracking methodology has recently been introduced for the detection, segmentation, and tracking of budding yeast cells in pre-recorded samples. Although this architecture has demonstrated a unique perspective on stable and consistent tracking, as well as missed instance re-interpolation, its evaluation has so far been largely confined to settings related to videomicroscopic environments. In this work, we aim to reveal the broader capabilities, advantages, and potential challenges of this architecture across various specifically designed scenarios, including a pedestrian tracking dataset. We also conduct an ablation study comparing the model against its restricted variants and the widely used Kalman filter. Furthermore, we present an attention analysis of the tracking architecture for both pretrained and non-pretrained models