Xiaotong Wang

Yuanhao Qu, Kaixuan Huang, Ming Yin et al.

The introduction of genome engineering technology has transformed biomedical research, making it possible to make precise changes to genetic information. However, creating an efficient gene-editing system requires a deep understanding of CRISPR technology, and the complex experimental systems under investigation. While Large Language Models (LLMs) have shown promise in various tasks, they often lack specific knowledge and struggle to accurately solve biological design problems. In this work, we introduce CRISPR-GPT, an LLM agent augmented with domain knowledge and external tools to automate and enhance the design process of CRISPR-based gene-editing experiments. CRISPR-GPT leverages the reasoning ability of LLMs to facilitate the process of selecting CRISPR systems, designing guide RNAs, recommending cellular delivery methods, drafting protocols, and designing validation experiments to confirm editing outcomes. We showcase the potential of CRISPR-GPT for assisting non-expert researchers with gene-editing experiments from scratch and validate the agent's effectiveness in a real-world use case. Furthermore, we explore the ethical and regulatory considerations associated with automated gene-editing design, highlighting the need for responsible and transparent use of these tools. Our work aims to bridge the gap between beginner biological researchers and CRISPR genome engineering techniques, and demonstrate the potential of LLM agents in facilitating complex biological discovery tasks. The published version of this draft is available at https://www.nature.com/articles/s41551-025-01463-z.

Le Cong, Zaixi Zhang, Xiaotong Wang et al.

Modern science advances fastest when thought meets action. LabOS represents the first AI co-scientist that unites computational reasoning with physical experimentation through multimodal perception, self-evolving agents, and Entended-Reality(XR)-enabled human-AI collaboration. By connecting multi-model AI agents, smart glasses, and human-AI collaboration, LabOS allows AI to see what scientists see, understand experimental context, and assist in real-time execution. Across applications--from cancer immunotherapy target discovery to stem-cell engineering -- LabOS shows that AI can move beyond computational design to participation, turning the laboratory into an intelligent, collaborative environment where human and machine discovery evolve together.

Yuan Tian, Chuhan Zhang, Xiaotong Wang et al.

Creating data reports is a labor-intensive task involving iterative data exploration, insight extraction, and narrative construction. A key challenge lies in composing the analysis logic-from defining objectives and transforming data to identifying and communicating insights. Manually crafting this logic can be cognitively demanding. While experienced analysts often reuse scripts from past projects, finding a perfect match for a new dataset is rare. Even when similar analyses are available online, they usually share only results or visualizations, not the underlying code, making reuse difficult. To address this, we present ReSpark, a system that leverages large language models (LLMs) to reverse-engineer analysis logic from existing reports and adapt it to new datasets. By generating draft analysis steps, ReSpark provides a warm start for users. It also supports interactive refinement, allowing users to inspect intermediate outputs, insert objectives, and revise content. We evaluate ReSpark through comparative and user studies, demonstrating its effectiveness in lowering the barrier to generating data reports without relying on existing analysis code.

Haowei Ni, Shuchen Meng, Xieming Geng et al.

Cardiovascular disease (CVD) is a leading cause of death globally, necessitating precise forecasting models for monitoring vital signs like heart rate, blood pressure, and ECG. Traditional models, such as ARIMA and Prophet, are limited by their need for manual parameter tuning and challenges in handling noisy, sparse, and highly variable medical data. This study investigates advanced deep learning models, including LSTM, and transformer-based architectures, for predicting heart rate time series from the MIT-BIH Database. Results demonstrate that deep learning models, particularly PatchTST, significantly outperform traditional models across multiple metrics, capturing complex patterns and dependencies more effectively. This research underscores the potential of deep learning to enhance patient monitoring and CVD management, suggesting substantial clinical benefits. Future work should extend these findings to larger, more diverse datasets and real-world clinical applications to further validate and optimize model performance.