Yaping Mao

h-index19
2papers
1,413citations

2 Papers

1.2DMMay 10, 2024
Perturbation results for distance-edge-monitoring numbers

Chenxu Yang, Ralf Klasing, Changxiang He et al.

Foucaud et al. recently introduced and initiated the study of a new graph-theoretic concept in the area of network monitoring. Given a graph $G=(V(G), E(G))$, a set $M \subseteq V(G)$ is a distance-edge-monitoring set if for every edge $e \in E(G)$, there is a vertex $x \in M$ and a vertex $y \in V(G)$ such that the edge $e$ belongs to all shortest paths between $x$ and $y$. The smallest size of such a set in $G$ is denoted by $\operatorname{dem}(G)$. Denoted by $G-e$ (resp. $G \backslash u$) the subgraph of $G$ obtained by removing the edge $e$ from $G$ (resp. a vertex $u$ together with all its incident edges from $G$). In this paper, we first show that $\operatorname{dem}(G-e)- \operatorname{dem}(G)\leq 2$ for any graph $G$ and edge $e \in E(G)$. Moreover, the bound is sharp. Next, we construct two graphs $G$ and $H$ to show that $\operatorname{dem}(G)-\operatorname{dem}(G\setminus u)$ and $\operatorname{dem}(H\setminus v)-\operatorname{dem}(H)$ can be arbitrarily large, where $u \in V(G)$ and $v \in V(H)$. We also study the relation between $\operatorname{dem}(H)$ and $\operatorname{dem}(G)$, where $H$ is a subgraph of $G$. In the end, we give an algorithm to judge whether the distance-edge monitoring set still remain in the resulting graph when any edge of the graph $G$ is deleted.

1.2QMJan 31, 2025Code
Single cell resolution 3D imaging and segmentation within intact live tissues

G. Paci, P. Vicente-Munuera, I. Fernandez-Mosquera et al.

Epithelial cells form diverse structures from squamous spherical organoids to densely packed pseudostratified tissues. Quantification of cellular properties in these contexts requires high-resolution deep imaging and computational techniques to achieve truthful three-dimensional (3D) structural features. Here, we describe a detailed step-by-step protocol for sample preparation, imaging and deep-learning-assisted cell segmentation to achieve accurate quantification of fluorescently labelled individual cells in 3D within live tissues. We share the lessons learned through troubleshooting 3D imaging of Drosophila wing discs, including considerations on the choice of microscopy modality and settings (objective, sample mounting) and available segmentation methods. In addition, we include a computational pipeline alongside custom code to assist replication of the protocol. While we focus on the segmentation of cell outlines from membrane labelling, this protocol applies to a wide variety of samples, and we believe it be valuable for studying other tissues that demand complex analysis in 3D.