Anton Nikolaev

Tianhao Zhang, Heather J. McCourty, Berardo M. Sanchez-Tafolla et al.

Deep learning has revolutionized medical and biological imaging, particularly in segmentation tasks. However, segmenting biological cells remains challenging due to the high variability and complexity of cell shapes. Addressing this challenge requires high-quality datasets that accurately represent the diverse morphologies found in biological cells. Existing cell segmentation datasets are often limited by their focus on regular and uniform shapes. In this paper, we introduce a novel benchmark dataset of Ntera-2 (NT2) cells, a pluripotent carcinoma cell line, exhibiting diverse morphologies across multiple stages of differentiation, capturing the intricate and heterogeneous cellular structures that complicate segmentation tasks. To address these challenges, we propose an uncertainty-aware deep learning framework for complex cellular morphology segmentation (MorphoSeg) by incorporating sampling of virtual outliers from low-likelihood regions during training. Our comprehensive experimental evaluations against state-of-the-art baselines demonstrate that MorphoSeg significantly enhances segmentation accuracy, achieving up to a 7.74% increase in the Dice Similarity Coefficient (DSC) and a 28.36% reduction in the Hausdorff Distance. These findings highlight the effectiveness of our dataset and methodology in advancing cell segmentation capabilities, especially for complex and variable cell morphologies. The dataset and source code is publicly available at https://github.com/RanchoGoose/MorphoSeg.

Moseli Mots'oehli, Anton Nikolaev, Wawan B. IGede et al.

Fish stock assessment often involves manual fish counting by taxonomy specialists, which is both time-consuming and costly. We propose FishNet, an automated computer vision system for both taxonomic classification and fish size estimation from images captured with a low-cost digital camera. The system first performs object detection and segmentation using a Mask R-CNN to identify individual fish from images containing multiple fish, possibly consisting of different species. Then each fish species is classified and the length is predicted using separate machine learning models. To develop the model, we use a dataset of 300,000 hand-labeled images containing 1.2M fish of 163 different species and ranging in length from 10cm to 250cm, with additional annotations and quality control methods used to curate high-quality training data. On held-out test data sets, our system achieves a 92% intersection over union on the fish segmentation task, a 89% top-1 classification accuracy on single fish species classification, and a 2.3cm mean absolute error on the fish length estimation task.