Gabriele Lagani

Gabriele Lagani, Fabrizio Falchi, Claudio Gennaro et al.

For a long time, biology and neuroscience fields have been a great source of inspiration for computer scientists, towards the development of Artificial Intelligence (AI) technologies. This survey aims at providing a comprehensive review of recent biologically-inspired approaches for AI. After introducing the main principles of computation and synaptic plasticity in biological neurons, we provide a thorough presentation of Spiking Neural Network (SNN) models, and we highlight the main challenges related to SNN training, where traditional backprop-based optimization is not directly applicable. Therefore, we discuss recent bio-inspired training methods, which pose themselves as alternatives to backprop, both for traditional and spiking networks. Bio-Inspired Deep Learning (BIDL) approaches towards advancing the computational capabilities and biological plausibility of current models.

Gabriele Lagani, Fabrizio Falchi, Claudio Gennaro et al.

Recently emerged technologies based on Deep Learning (DL) achieved outstanding results on a variety of tasks in the field of Artificial Intelligence (AI). However, these encounter several challenges related to robustness to adversarial inputs, ecological impact, and the necessity of huge amounts of training data. In response, researchers are focusing more and more interest on biologically grounded mechanisms, which are appealing due to the impressive capabilities exhibited by biological brains. This survey explores a range of these biologically inspired models of synaptic plasticity, their application in DL scenarios, and the connections with models of plasticity in Spiking Neural Networks (SNNs). Overall, Bio-Inspired Deep Learning (BIDL) represents an exciting research direction, aiming at advancing not only our current technologies but also our understanding of intelligence.

Gabriele Lagani, Claudio Gennaro, Hannes Fassold et al.

Learning algorithms for Deep Neural Networks are typically based on supervised end-to-end Stochastic Gradient Descent (SGD) training with error backpropagation (backprop). Backprop algorithms require a large number of labelled training samples to achieve high performance. However, in many realistic applications, even if there is plenty of image samples, very few of them are labelled, and semi-supervised sample-efficient training strategies have to be used. Hebbian learning represents a possible approach towards sample efficient training; however, in current solutions, it does not scale well to large datasets. In this paper, we present FastHebb, an efficient and scalable solution for Hebbian learning which achieves higher efficiency by 1) merging together update computation and aggregation over a batch of inputs, and 2) leveraging efficient matrix multiplication algorithms on GPU. We validate our approach on different computer vision benchmarks, in a semi-supervised learning scenario. FastHebb outperforms previous solutions by up to 50 times in terms of training speed, and notably, for the first time, we are able to bring Hebbian algorithms to ImageNet scale.

Gabriele Lagani, Davide Bacciu, Claudio Gallicchio et al.

Features extracted from Deep Neural Networks (DNNs) have proven to be very effective in the context of Content Based Image Retrieval (CBIR). In recent work, biologically inspired \textit{Hebbian} learning algorithms have shown promises for DNN training. In this contribution, we study the performance of such algorithms in the development of feature extractors for CBIR tasks. Specifically, we consider a semi-supervised learning strategy in two steps: first, an unsupervised pre-training stage is performed using Hebbian learning on the image dataset; second, the network is fine-tuned using supervised Stochastic Gradient Descent (SGD) training. For the unsupervised pre-training stage, we explore the nonlinear Hebbian Principal Component Analysis (HPCA) learning rule. For the supervised fine-tuning stage, we assume sample efficiency scenarios, in which the amount of labeled samples is just a small fraction of the whole dataset. Our experimental analysis, conducted on the CIFAR10 and CIFAR100 datasets shows that, when few labeled samples are available, our Hebbian approach provides relevant improvements compared to various alternative methods.

Luca Ciampi, Ludovico Iannello, Fabrizio Tonelli et al.

In this paper, we present a neuro-inspired approach to reservoir computing (RC) in which a network of in vitro cultured cortical neurons serves as the physical reservoir. Rather than relying on artificial recurrent models to approximate neural dynamics, our biological reservoir computing (BRC) system leverages the spontaneous and stimulus-evoked activity of living neural circuits as its computational substrate. A high-density multi-electrode array (HD-MEA) provides simultaneous stimulation and readout across hundreds of channels: input patterns are delivered through selected electrodes, while the remaining ones capture the resulting high-dimensional neural responses, yielding a biologically grounded feature representation. A linear readout layer (single-layer perceptron) is then trained to classify these reservoir states, enabling the living neural network to perform static visual pattern-recognition tasks within a computer-vision framework. We evaluate the system across a sequence of tasks of increasing difficulty, ranging from pointwise stimuli to oriented bars, clock-digit-like shapes, and handwritten digits from the MNIST dataset. Despite the inherent variability of biological neural responses-arising from noise, spontaneous activity, and inter-session differences-the system consistently generates high-dimensional representations that support accurate classification. These results demonstrate that in vitro cortical networks can function as effective reservoirs for static visual pattern recognition, opening new avenues for integrating living neural substrates into neuromorphic computing frameworks. More broadly, this work contributes to the effort to incorporate biological principles into machine learning and supports the goals of neuro-inspired vision by illustrating how living neural systems can inform the design of efficient and biologically grounded computational models.

Luca Ciampi, Gabriele Lagani, Giuseppe Amato et al.

We propose a novel bio-inspired semi-supervised learning approach for training downsampling-upsampling semantic segmentation architectures. The first stage does not use backpropagation. Rather, it exploits the Hebbian principle ``fire together, wire together'' as a local learning rule for updating the weights of both convolutional and transpose-convolutional layers, allowing unsupervised discovery of data features. In the second stage, the model is fine-tuned with standard backpropagation on a small subset of labeled data. We evaluate our methodology through experiments conducted on several widely used biomedical datasets, deeming that this domain is paramount in computer vision and is notably impacted by data scarcity. Results show that our proposed method outperforms SOTA approaches across different levels of label availability. Furthermore, we show that using our unsupervised stage to initialize the SOTA approaches leads to performance improvements. The code to replicate our experiments can be found at https://github.com/ciampluca/hebbian-bootstraping-semi-supervised-medical-imaging

Luca Ciampi, Gabriele Lagani, Giuseppe Amato et al.

Supervised deep learning for semantic segmentation has achieved excellent results in accurately identifying anatomical and pathological structures in medical images. However, it often requires large annotated training datasets, which limits its scalability in clinical settings. To address this challenge, semi-supervised learning is a well-established approach that leverages both labeled and unlabeled data. In this paper, we introduce a novel semi-supervised teacher-student framework for biomedical image segmentation, inspired by the recent success of generative models. Our approach leverages denoising diffusion probabilistic models (DDPMs) to generate segmentation masks by progressively refining noisy inputs conditioned on the corresponding images. The teacher model is first trained in an unsupervised manner using a cycle-consistency constraint based on noise-corrupted image reconstruction, enabling it to generate informative semantic masks. Subsequently, the teacher is integrated into a co-training process with a twin-student network. The student learns from ground-truth labels when available and from teacher-generated pseudo-labels otherwise, while the teacher continuously improves its pseudo-labeling capabilities. Finally, to further enhance performance, we introduce a multi-round pseudo-label generation strategy that iteratively improves the pseudo-labeling process. We evaluate our approach on multiple biomedical imaging benchmarks, spanning multiple imaging modalities and segmentation tasks. Experimental results show that our method consistently outperforms state-of-the-art semi-supervised techniques, highlighting its effectiveness in scenarios with limited annotated data. The code to replicate our experiments can be found at https://github.com/ciampluca/diffusion_semi_supervised_biomedical_image_segmentation

Ludovico Iannello, Luca Ciampi, Gabriele Lagani et al.

In this paper, we introduce a paradigm for reservoir computing (RC) that leverages a pool of cultured biological neurons as the reservoir substrate, creating a biological reservoir computing (BRC). This system operates similarly to an echo state network (ESN), with the key distinction that the neural activity is generated by a network of cultured neurons, rather than being modeled by traditional artificial computational units. The neuronal activity is recorded using a multi-electrode array (MEA), which enables high-throughput recording of neural signals. In our approach, inputs are introduced into the network through a subset of the MEA electrodes, while the remaining electrodes capture the resulting neural activity. This generates a nonlinear mapping of the input data to a high-dimensional biological feature space, where distinguishing between data becomes more efficient and straightforward, allowing a simple linear classifier to perform pattern recognition tasks effectively. To evaluate the performance of our proposed system, we present an experimental study that includes various input patterns, such as positional codes, bars with different orientations, and a digit recognition task. The results demonstrate the feasibility of using biological neural networks to perform tasks traditionally handled by artificial neural networks, paving the way for further exploration of biologically-inspired computing systems, with potential applications in neuromorphic engineering and bio-hybrid computing.

Ludovico Iannello, Luca Ciampi, Fabrizio Tonelli et al.

In this paper, we present a biologically grounded approach to reservoir computing (RC), in which a network of cultured biological neurons serves as the reservoir substrate. This system, referred to as biological reservoir computing (BRC), replaces artificial recurrent units with the spontaneous and evoked activity of living neurons. A multi-electrode array (MEA) enables simultaneous stimulation and readout across multiple sites: inputs are delivered through a subset of electrodes, while the remaining ones capture the resulting neural responses, mapping input patterns into a high-dimensional biological feature space. We evaluate the system through a case study on digit classification using a custom dataset. Input images are encoded and delivered to the biological reservoir via electrical stimulation, and the corresponding neural activity is used to train a simple linear classifier. To contextualize the performance of the biological system, we also include a comparison with a standard artificial reservoir trained on the same task. The results indicate that the biological reservoir can effectively support classification, highlighting its potential as a viable and interpretable computational substrate. We believe this work contributes to the broader effort of integrating biological principles into machine learning and aligns with the goals of human-inspired vision by exploring how living neural systems can inform the design of efficient and biologically plausible models.

Teodor Boyadzhiev, Gabriele Lagani, Luca Ciampi et al.

The integration of computer vision and deep learning is an essential part of documenting and preserving cultural heritage, as well as improving visitor experiences. In recent years, two deep learning paradigms have been established in the field of computer vision: convolutional neural networks and transformer architectures. The present study aims to make a comparative analysis of some representatives of these two techniques of their ability to transfer knowledge from generic dataset, such as ImageNet, to cultural heritage specific tasks. The results of testing examples of the architectures VGG, ResNet, DenseNet, Visual Transformer, Swin Transformer, and PoolFormer, showed that DenseNet is the best in terms of efficiency-computability ratio.

Gabriele Lagani, Fabrizio Falchi, Claudio Gennaro et al.

We propose to address the issue of sample efficiency, in Deep Convolutional Neural Networks (DCNN), with a semi-supervised training strategy that combines Hebbian learning with gradient descent: all internal layers (both convolutional and fully connected) are pre-trained using an unsupervised approach based on Hebbian learning, and the last fully connected layer (the classification layer) is trained using Stochastic Gradient Descent (SGD). In fact, as Hebbian learning is an unsupervised learning method, its potential lies in the possibility of training the internal layers of a DCNN without labels. Only the final fully connected layer has to be trained with labeled examples. We performed experiments on various object recognition datasets, in different regimes of sample efficiency, comparing our semi-supervised (Hebbian for internal layers + SGD for the final fully connected layer) approach with end-to-end supervised backprop training, and with semi-supervised learning based on Variational Auto-Encoder (VAE). The results show that, in regimes where the number of available labeled samples is low, our semi-supervised approach outperforms the other approaches in almost all the cases.

Gabriele Lagani, Giuseppe Amato, Fabrizio Falchi et al.

Recent work has shown that biologically plausible Hebbian learning can be integrated with backpropagation learning (backprop), when training deep convolutional neural networks. In particular, it has been shown that Hebbian learning can be used for training the lower or the higher layers of a neural network. For instance, Hebbian learning is effective for re-training the higher layers of a pre-trained deep neural network, achieving comparable accuracy w.r.t. SGD, while requiring fewer training epochs, suggesting potential applications for transfer learning. In this paper we build on these results and we further improve Hebbian learning in these settings, by using a nonlinear Hebbian Principal Component Analysis (HPCA) learning rule, in place of the Hebbian Winner Takes All (HWTA) strategy used in previous work. We test this approach in the context of computer vision. In particular, the HPCA rule is used to train Convolutional Neural Networks in order to extract relevant features from the CIFAR-10 image dataset. The HPCA variant that we explore further improves the previous results, motivating further interest towards biologically plausible learning algorithms.

Gabriele Lagani, Raffaele Mazziotti, Fabrizio Falchi et al.

Previous work has shown that it is possible to train neuronal cultures on Multi-Electrode Arrays (MEAs), to recognize very simple patterns. However, this work was mainly focused to demonstrate that it is possible to induce plasticity in cultures, rather than performing a rigorous assessment of their pattern recognition performance. In this paper, we address this gap by developing a methodology that allows us to assess the performance of neuronal cultures on a learning task. Specifically, we propose a digital model of the real cultured neuronal networks; we identify biologically plausible simulation parameters that allow us to reliably reproduce the behavior of real cultures; we use the simulated culture to perform handwritten digit recognition and rigorously evaluate its performance; we also show that it is possible to find improved simulation parameters for the specific task, which can guide the creation of real cultures.