An‐Ping Zeng

Jiangbin Zheng, Han Zhang, Qianqing Xu et al.

Enzyme design plays a crucial role in both industrial production and biology. However, this field faces challenges due to the lack of comprehensive benchmarks and the complexity of enzyme design tasks, leading to a dearth of systematic research. Consequently, computational enzyme design is relatively overlooked within the broader protein domain and remains in its early stages. In this work, we address these challenges by introducing MetaEnzyme, a staged and unified enzyme design framework. We begin by employing a cross-modal structure-to-sequence transformation architecture, as the feature-driven starting point to obtain initial robust protein representation. Subsequently, we leverage domain adaptive techniques to generalize specific enzyme design tasks under low-resource conditions. MetaEnzyme focuses on three fundamental low-resource enzyme redesign tasks: functional design (FuncDesign), mutation design (MutDesign), and sequence generation design (SeqDesign). Through novel unified paradigm and enhanced representation capabilities, MetaEnzyme demonstrates adaptability to diverse enzyme design tasks, yielding outstanding results. Wet lab experiments further validate these findings, reinforcing the efficacy of the redesign process.

Chaorong Li, Malu Zhang, Wei Huang et al.

Deep Convolutional Neural Network (DCNN) and Transformer have achieved remarkable successes in image recognition. However, their performance in fine-grained image recognition is still difficult to meet the requirements of actual needs. This paper proposes a Sequence Random Network (SRN) to enhance the performance of DCNN. The output of DCNN is one-dimensional features. This one-dimensional feature abstractly represents image information, but it does not express well the detailed information of image. To address this issue, we use the proposed SRN which composed of BiLSTM and several Tanh-Dropout blocks (called BiLSTM-TDN), to further process DCNN one-dimensional features for highlighting the detail information of image. After the feature transform by BiLSTM-TDN, the recognition performance has been greatly improved. We conducted the experiments on six fine-grained image datasets. Except for FGVC-Aircraft, the accuracies of the proposed methods on the other datasets exceeded 99%. Experimental results show that BiLSTM-TDN is far superior to the existing state-of-the-art methods. In addition to DCNN, BiLSTM-TDN can also be extended to other models, such as Transformer.