Gaoyang Li

Ping Xu, Zaitian Wang, Zhirui Wang et al.

Single-cell RNA sequencing (scRNA-seq) technology enables systematic delineation of cellular states and interactions, providing crucial insights into cellular heterogeneity. Building on this potential, numerous computational methods have been developed for tasks such as cell clustering, cell type annotation, and marker gene identification. To fully assess and compare these methods, standardized, analysis-ready datasets are essential. However, such datasets remain scarce, and variations in data formats, preprocessing workflows, and annotation strategies hinder reproducibility and complicate systematic evaluation of existing methods. To address these challenges, we present scUnified, an AI-ready standardized resource for single-cell RNA sequencing data that consolidates 13 high-quality datasets spanning two species (human and mouse) and nine tissue types. All datasets undergo standardized quality control and preprocessing and are stored in a uniform format to enable direct application in diverse computational analyses without additional data cleaning. We further demonstrate the utility of scUnified through experimental analyses of representative biological tasks, providing a reproducible foundation for the standardized evaluation of computational methods on a unified dataset.

Gaoyang Li, Jinyu Yang, Chunguo Wu et al.

Support vector regression (SVR) is one of the most popular machine learning algorithms aiming to generate the optimal regression curve through maximizing the minimal margin of selected training samples, i.e., support vectors. Recent researchers reveal that maximizing the margin distribution of whole training dataset rather than the minimal margin of a few support vectors, is prone to achieve better generalization performance. However, the margin distribution support vector regression machines suffer difficulties resulted from solving a non-convex quadratic optimization, compared to the margin distribution strategy for support vector classification, This paper firstly proposes a maximal margin distribution model for SVR(MMD-SVR), then implementing coupled constrain factor to convert the non-convex quadratic optimization to a convex problem with linear constrains, which enhance the training feasibility and efficiency for SVR to derived from maximizing the margin distribution. The theoretical and empirical analysis illustrates the superiority of MMD-SVR. In addition, numerical experiments show that MMD-SVR could significantly improve the accuracy of prediction and generate more smooth regression curve with better generalization compared with the classic SVR.