Alireza Naghizadeh

Xiang Zhang, Boxuan Zhang, Alireza Naghizadeh et al.

Chimeric antigen receptor (CAR)-T and NK cell immunotherapies have transformed cancer treatment, and recent studies suggest that the quality of the CAR-T/NK cell immunological synapse (IS) may serve as a functional biomarker for predicting therapeutic efficacy. Accurate detection and segmentation of CAR-T/NK IS structures using artificial neural networks (ANNs) can greatly increase the speed and reliability of IS quantification. However, a persistent challenge is the limited size of annotated microscopy datasets, which restricts the ability of ANNs to generalize. To address this challenge, we integrate two complementary data-augmentation frameworks. First, we employ Instance Aware Automatic Augmentation (IAAA), an automated, instance-preserving augmentation method that generates synthetic CAR-T/NK IS images and corresponding segmentation masks by applying optimized augmentation policies to original IS data. IAAA supports multiple imaging modalities (e.g., fluorescence and brightfield) and can be applied directly to CAR-T/NK IS images derived from patient samples. In parallel, we introduce a Semantic-Aware AI Augmentation (SAAA) pipeline that combines a diffusion-based mask generator with a Pix2Pix conditional image synthesizer. This second method enables the creation of diverse, anatomically realistic segmentation masks and produces high-fidelity CAR-T/NK IS images aligned with those masks, further expanding the training corpus beyond what IAAA alone can provide. Together, these augmentation strategies generate synthetic images whose visual and structural properties closely match real IS data, significantly improving CAR-T/NK IS detection and segmentation performance. By enhancing the robustness and accuracy of IS quantification, this work supports the development of more reliable imaging-based biomarkers for predicting patient response to CAR-T/NK immunotherapy.

Alireza Naghizadeh, Mohammadsajad Abavisani, Dimitris N. Metaxas

A major problem in data augmentation is to ensure that the generated new samples cover the search space. This is a challenging problem and requires exploration for data augmentation policies to ensure their effectiveness in covering the search space. In this paper, we propose Greedy AutoAugment as a highly efficient search algorithm to find the best augmentation policies. We use a greedy approach to reduce the exponential growth of the number of possible trials to linear growth. The Greedy Search also helps us to lead the search towards the sub-policies with better results, which eventually helps to increase the accuracy. The proposed method can be used as a reliable addition to the current artifitial neural networks. Our experiments on four datasets (Tiny ImageNet, CIFAR-10, CIFAR-100, and SVHN) show that Greedy AutoAugment provides better accuracy, while using 360 times fewer computational resources.

Alireza Naghizadeh, Tahereh Yourdkhani, Behrooz Razeghi et al.

Peer-to-Peer (P2P) networks as distributed solutions are used in a variety of applications. Based on the type of routing for queries among their nodes, they are classified into three groups: structured, unstructured and small-world P2P networks. Each of these categories has its own applications and benefits. Structured networks by using Distributed Hash Tables (DHT) can forward request search queries more efficiently. These networks usually organize a specific topology and make a geometrical shape. A circular topology is a prevalent design which was first introduced by Chord. In this paper, we propose BSROne, a circular structured P2P design which attempts to consider several shortcomings in the current networks. In our proposed method, we want to achieve O(1) routing time without requiring all of the nodes to know about each other. By removing the real connections between nodes and tying all of them with super-nodes, we could reduce the number of overheads that are essential to maintain the connectivity between nodes in such networks. Furthermore, we gave the network an ability to scale up by introducing one layer above super-nodes. We achieved this by emulating the design of binary search algorithm for supreme-nodes. In this paper, at first we introduce a design where fixed super-nodes with unlimited resources are given to the distributed network. In the next step, we explain how it can manage to work as a P2P application. We finally discuss the possibility of removing the scalability issue in a P2P environment for our design.