Intracellular regulatory control of gene expression process
For synthetic biology researchers, this work proposes novel genetic circuit designs for feedback control, but it is incremental as it only provides simulation results without experimental validation or quantitative benchmarks.
The paper designs intracellular feedback control strategies using genetic comparators (Genetic Relay Switch, Shifted Subtractor, Bistable Comparator) to regulate gene expression, with numerical simulations demonstrating effectiveness.
This paper presents an intracellular feedback control strategy, to regulate the gene expression process dynamics. For this purpose, two types of genetic circuits are designed in order to compare concentrations of the input transcription factor (the desired input) and the protein produced by the expression of target gene (process output). Genetic Relay Switch and Shifted Subtractor are proposed and employed to design Discrete Comparator. Also, another type of genetic comparator is presented named Bistable Comparator. These synthetic comparators are used to produce the required amount of the protein that activates the expression of the target gene. Numerical simulation results, demonstrate the effectiveness of the designed controllers.