A new solution to the curved Ewald sphere problem for 3D image reconstruction in electron microscopy
This work solves a reconstruction problem for cryo-electron microscopy, enabling higher resolution imaging of larger samples.
This paper presents an algorithm for 3D image reconstruction from 2D images, specifically addressing the challenge posed by the curvature of the Ewald sphere, which prevents the 2D images from being approximated as simple projections of the object. This method is particularly relevant for cryo-electron microscopy applications involving larger samples, higher resolution, or lower energy electron beams.
We develop an algorithm capable of imaging a three-dimensional object given a collection of two-dimensional images of that object that are significantly influenced by the curvature of the Ewald sphere. These two-dimensional images cannot be approximated as projections of the object. Such an algorithm is useful in cryo-electron microscopy where larger samples, higher resolution, or lower energy electron beams are desired, all of which contribute to the significance of Ewald curvature.