In vivo learning-based control of microbial populations density in bioreactors
This work addresses the challenge of maintaining optimal microbial density for bio-factory applications, representing an incremental step toward controlling more complex microbial consortia.
The authors tackled the problem of regulating bacterial population density in bioreactors by developing a learning-based control strategy using a sim-to-real paradigm, which achieved similar performance to traditional controllers like PI and MPC in vivo tests.
A key problem toward the use of microorganisms as bio-factories is reaching and maintaining cellular communities at a desired density and composition so that they can efficiently convert their biomass into useful compounds. Promising technological platforms for the real time, scalable control of cellular density are bioreactors. In this work, we developed a learning-based strategy to expand the toolbox of available control algorithms capable of regulating the density of a \textit{single} bacterial population in bioreactors. Specifically, we used a sim-to-real paradigm, where a simple mathematical model, calibrated using a few data, was adopted to generate synthetic data for the training of the controller. The resulting policy was then exhaustively tested in vivo using a low-cost bioreactor known as Chi.Bio, assessing performance and robustness. In addition, we compared the performance with more traditional controllers (namely, a PI and an MPC), confirming that the learning-based controller exhibits similar performance in vivo. Our work showcases the viability of learning-based strategies for the control of cellular density in bioreactors, making a step forward toward their use for the control of the composition of microbial consortia.