Resolution Enhancement of Under-sampled Photoacoustic Microscopy Images using Implicit Neural Representations
This work addresses resolution enhancement for subcutaneous vascular imaging in photoacoustic microscopy, offering a novel method that is incremental but improves upon traditional deconvolution and interpolation techniques.
The paper tackled the problem of low spatial resolution and long scanning times in Acoustic-Resolution Photoacoustic Microscopy (AR-PAM) by proposing an Implicit Neural Representations (INR) approach, which improved resolution by learning a continuous mapping and treating the PSF as a learnable parameter, resulting in significant gains in PSNR and SSIM on simulated data and sharper images in real-world experiments.
Acoustic-Resolution Photoacoustic Microscopy (AR-PAM) is promising for subcutaneous vascular imaging, but its spatial resolution is constrained by the Point Spread Function (PSF). Traditional deconvolution methods like Richardson-Lucy and model-based deconvolution use the PSF to improve resolution. However, accurately measuring the PSF is difficult, leading to reliance on less accurate blind deconvolution techniques. Additionally, AR-PAM suffers from long scanning times, which can be reduced via down-sampling, but this necessitates effective image recovery from under-sampled data, a task where traditional interpolation methods fall short, particularly at high under-sampling rates. To address these challenges, we propose an approach based on Implicit Neural Representations (INR). This method learns a continuous mapping from spatial coordinates to initial acoustic pressure, overcoming the limitations of discrete imaging and enhancing AR-PAM's resolution. By treating the PSF as a learnable parameter within the INR framework, our technique mitigates inaccuracies associated with PSF estimation. We evaluated our method on simulated vascular data, showing significant improvements in Peak Signal-to-Noise Ratio (PSNR) and Structural Similarity Index (SSIM) over conventional methods. Qualitative enhancements were also observed in leaf vein and in vivo mouse brain microvasculature images. When applied to a custom AR-PAM system, experiments with pencil lead demonstrated that our method delivers sharper, higher-resolution results, indicating its potential to advance photoacoustic microscopy.