trAIce3D: A Prompt-Driven Transformer Based U-Net for Semantic Segmentation of Microglial Cells from Large-Scale 3D Microscopy Images
This addresses the need for precise segmentation of microglial cells in neurobiological research, enabling scalable analysis of cellular morphologies for insights into neurodegenerative diseases, though it is incremental as it builds on existing U-Net and transformer methods.
The paper tackles the problem of segmenting microglial cells from large-scale 3D microscopy images, which is challenging due to overlapping structures and noise, and introduces trAIce3D, a deep-learning architecture that improves segmentation accuracy and generalization, as demonstrated on a dataset of 41,230 cells.
The shape of a cell contains essential information about its function within the biological system. Segmenting these structures from large-scale 3D microscopy images is challenging, limiting clinical insights especially for microglia, immune-associated cells involved in neurodegenerative diseases. Existing segmentation methods mainly focus on cell bodies, struggle with overlapping structures, perform poorly on noisy images, require hyperparameter tuning for each new dataset, or rely on tedious semi-automated approaches. We introduce trAIce3D, a deep-learning architecture designed for precise microglia segmentation, capturing both somas and branches. It employs a two-stage approach: first, a 3D U-Net with vision transformers in the encoder detects somas using a sliding-window technique to cover the entire image. Then, the same architecture, enhanced with cross-attention blocks in skip connections, refines each soma and its branches by using soma coordinates as a prompt and a 3D window around the target cell as input. Training occurs in two phases: self-supervised Soma Segmentation, followed by prompt-based Branch Segmentation, leveraging pre-trained weights from the first phase. Trained and evaluated on a dataset of 41,230 microglial cells, trAIce3D significantly improves segmentation accuracy and generalization, enabling scalable analysis of complex cellular morphologies. While optimized for microglia, its architecture can extend to other intricate cell types, such as neurons and astrocytes, broadening its impact on neurobiological research.