Juergen W. Czarske

Jiawei Sun, Bin Zhao, Dong Wang et al.

Quantitative phase imaging (QPI) through multi-core fibers (MCFs) has been an emerging in vivo label-free endoscopic imaging modality with minimal invasiveness. However, the computational demands of conventional iterative phase retrieval algorithms have limited their real-time imaging potential. We demonstrate a learning-based MCF phase imaging method, that significantly reduced the phase reconstruction time to 5.5 ms, enabling video-rate imaging at 181 fps. Moreover, we introduce an innovative optical system that automatically generated the first open-source dataset tailored for MCF phase imaging, comprising 50,176 paired speckle and phase images. Our trained deep neural network (DNN) demonstrates robust phase reconstruction performance in experiments with a mean fidelity of up to 99.8\%. Such an efficient fiber phase imaging approach can broaden the applications of QPI in hard-to-reach areas.

Jiawei Sun, Bin Yang, Nektarios Koukourakis et al.

Optical tomography has emerged as a non-invasive imaging method, providing three-dimensional insights into subcellular structures and thereby enabling a deeper understanding of cellular functions, interactions, and processes. Conventional optical tomography methods are constrained by a limited illumination scanning range, leading to anisotropic resolution and incomplete imaging of cellular structures. To overcome this problem, we employ a compact multi-core fibre-optic cell rotator system that facilitates precise optical manipulation of cells within a microfluidic chip, achieving full-angle projection tomography with isotropic resolution. Moreover, we demonstrate an AI-driven tomographic reconstruction workflow, which can be a paradigm shift from conventional computational methods, often demanding manual processing, to a fully autonomous process. The performance of the proposed cell rotation tomography approach is validated through the three-dimensional reconstruction of cell phantoms and HL60 human cancer cells. The versatility of this learning-based tomographic reconstruction workflow paves the way for its broad application across diverse tomographic imaging modalities, including but not limited to flow cytometry tomography and acoustic rotation tomography. Therefore, this AI-driven approach can propel advancements in cell biology, aiding in the inception of pioneering therapeutics, and augmenting early-stage cancer diagnostics.