Light-field Microscopy with a Consumer Light-field Camera
This work addresses a cost-effective solution for light-field microscopy in domains such as quality control and surface characterization, but it is incremental as it adapts existing technology rather than introducing a new method.
The paper tackled the problem of using an inexpensive consumer light-field camera (Lytro) for light-field microscopy by adapting its optical system to prevent information loss, achieving a maximum spatial resolution of about 6.25μm for low-magnification applications like quality control.
We explore the use of inexpensive consumer light- field camera technology for the purpose of light-field mi- croscopy. Our experiments are based on the Lytro (first gen- eration) camera. Unfortunately, the optical systems of the Lytro and those of microscopes are not compatible, lead- ing to a loss of light-field information due to angular and spatial vignetting when directly recording microscopic pic- tures. We therefore consider an adaptation of the Lytro op- tical system. We demonstrate that using the Lytro directly as an oc- ular replacement, leads to unacceptable spatial vignetting. However, we also found a setting that allows the use of the Lytro camera in a virtual imaging mode which prevents the information loss to a large extent. We analyze the new vir- tual imaging mode and use it in two different setups for im- plementing light-field microscopy using a Lytro camera. As a practical result, we show that the camera can be used for low magnification work, as e.g. common in quality control, surface characterization, etc. We achieve a maximum spa- tial resolution of about 6.25μm, albeit at a limited SNR for the side views.