DEEP$^2$: Deep Learning Powered De-scattering with Excitation Patterning
This addresses a bottleneck in biomedical imaging for researchers, though it is incremental as it builds on the existing DEEP method.
The paper tackles the limited throughput in deep-tissue imaging by introducing DEEP^2, a deep learning model that reduces the required patterned excitations from hundreds to tens, improving throughput by almost an order of magnitude in in-vivo experiments.
Limited throughput is a key challenge in in-vivo deep-tissue imaging using nonlinear optical microscopy. Point scanning multiphoton microscopy, the current gold standard, is slow especially compared to the wide-field imaging modalities used for optically cleared or thin specimens. We recently introduced 'De-scattering with Excitation Patterning or DEEP', as a widefield alternative to point-scanning geometries. Using patterned multiphoton excitation, DEEP encodes spatial information inside tissue before scattering. However, to de-scatter at typical depths, hundreds of such patterned excitations are needed. In this work, we present DEEP$^2$, a deep learning based model, that can de-scatter images from just tens of patterned excitations instead of hundreds. Consequently, we improve DEEP's throughput by almost an order of magnitude. We demonstrate our method in multiple numerical and physical experiments including in-vivo cortical vasculature imaging up to four scattering lengths deep, in alive mice.