CellINR: Implicitly Overcoming Photo-induced Artifacts in 4D Live Fluorescence Microscopy
This addresses image quality issues for biological researchers using live cell microscopy, offering incremental improvements in reconstruction methods.
The paper tackles photo-induced artifacts like photobleaching in 4D live fluorescence microscopy, proposing CellINR to improve artifact removal and structural continuity, with experimental results showing it significantly outperforms existing techniques.
4D live fluorescence microscopy is often compromised by prolonged high intensity illumination which induces photobleaching and phototoxic effects that generate photo-induced artifacts and severely impair image continuity and detail recovery. To address this challenge, we propose the CellINR framework, a case-specific optimization approach based on implicit neural representation. The method employs blind convolution and structure amplification strategies to map 3D spatial coordinates into the high frequency domain, enabling precise modeling and high-accuracy reconstruction of cellular structures while effectively distinguishing true signals from artifacts. Experimental results demonstrate that CellINR significantly outperforms existing techniques in artifact removal and restoration of structural continuity, and for the first time, a paired 4D live cell imaging dataset is provided for evaluating reconstruction performance, thereby offering a solid foundation for subsequent quantitative analyses and biological research. The code and dataset will be public.