Towards a Frugal Photosynthesis Sensing Toolkit for Data-Driven Plant Science Education and Exploration
This work provides an accessible tool for studying photosynthetic dynamics, addressing the need for frugal sensing in plant science education and exploration.
PhytoBits is a low-cost, open-source gas-exchange sensing toolkit that distinguishes C3, CAM, facultative CAM, and developmental CAM photosynthetic pathways, validated against research-grade systems. It enables multi-day monitoring of plant gas-exchange for education and research.
Rapid environmental change and advances in data-driven analysis highlight the need not only to use computational tools, but also to foster understanding of the natural world and inspire creativity. Photosynthesis, the process that fuels nearly all life on Earth, provides a compelling context for such learning, particularly in understanding how plants alter their photosynthetic strategies in response to environmental changes. However, existing tools for studying photosynthesis are often inaccessible or limited to demonstrating its presence, rather than capturing its temporal dynamics. We present PhytoBits, a frugal in situ gas-exchange sensing toolkit for distinguishing and teaching photosynthetic strategies. PhytoBits combines leaf enclosure with accessible materials, an off-the-shelf CO\textsubscript{2} sensor, and a low-cost microcontroller, to support multi-day monitoring of plant gas-exchange in educational and research contexts. We validated PhytoBits against research-grade gas-exchange systems, confirming that it identifies C\textsubscript{3} and CAM (Crassulacean Acid Metabolism) photosynthetic pathways. In addition to obligate CAM, PhytoBits also resolves facultative CAM and developmental CAM dynamics in plants. This work presents an early-stage hardware validation; user deployment studies, open-source code dissemination, and automated pathway classification are planned as future work.